Abstract | OBJECTIVE: SAMPLE POPULATION: 293 plasma samples submitted for analysis of canine vWF antigen (vWF:Ag) and 12 control plasma samples from dogs with inherited type 2 or 3 vWD. PROCEDURE: Bovine collagens were evaluated for suitability as binding substrate for vWF. Assay sensitivity to depletion, proteolytic degradation, or a genetic deficiency of high-molecular-weight vWF were determined. Amounts of vWF:Ag and vWF:CBA were measured. The ratio of vWF:Ag to vWF:CBA was used to discriminate between type 1 and type 2 vWD. RESULTS: An assay for canine vWF activity was developed by use of mixed collagen (types I and III). When vWF:Ag was used to subtype vWD, 48% of the dogs were classified as clinically normal, 9% as indeterminate, and 43% as type 1 vWD. Inclusion of vWF activity resulted in reclassification of 5% of those identified as type 1 to type 2 vWD. However, vWF:CBA of the reclassified dogs was not persistently abnormal, a finding compatible with acquired type 2 vWD. Some Doberman Pinschers had lower antigen-to-activity ratios than other breeds with type 1 vWD, suggesting that Doberman Pinschers have more functional circulating vWF. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of canine vWF activity should be included among the vWF-specific assays used to confirm type 2 vWD. The prevalence of inherited forms of type 2 vWD in screened dogs is lower than acquired forms that can result secondary to underlying disease.
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Authors | Elizabeth Peet Sabino, Hollis N Erb, James L Catalfamo |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 67
Issue 2
Pg. 242-9
(Feb 2006)
ISSN: 0002-9645 [Print] United States |
PMID | 16454628
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- von Willebrand Factor
- Collagen
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Topics |
- Animals
- Collagen
(metabolism)
- Dog Diseases
(blood, diagnosis, metabolism)
- Dogs
- Enzyme-Linked Immunosorbent Assay
- Female
- Male
- Sensitivity and Specificity
- von Willebrand Diseases
(blood, diagnosis, metabolism, veterinary)
- von Willebrand Factor
(analysis, metabolism)
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