Expression of oxidative stress-related molecules in circulating leukocytes and urine in patients with chronic viral hepatitis.

Abstract

AIMS:

Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress-related molecules remains to be clarified.

METHODS:

4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), catalase, superoxide dismutase-1 (SOD-1), glutathione peroxidase-1, thioredoxin (TRX) in leukocytes and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) were examined in 164 persons, including 130 chronic viral hepatitis patients and 34 normal individuals, by Western blot analysis and enzyme-linked immunosorbent assay, respectively. Hepatic expression of these proteins was immunohistochemically examined in 12 patients with chronic viral hepatitis, compared with three persons without liver damage.

RESULTS:

The 4-HNE/beta-actin ratios in chronic viral hepatitis were significantly higher than those in normal individuals (P<0.01), and were significantly correlated with asparate aminotransferase (AST) and alanine aminotransferase (ALT) (P<0.01, each). The catalase/beta-actin and SOD-1/beta-actin ratios in chronic viral hepatitis were higher than those in normal individuals, and were significantly correlated with 4-HNE/beta-actin ratios (P<0.01, each). Hepatic expression of 4-HNE, 4-HHE, catalase, SOD-1 and TRX in chronic viral hepatitis was higher than that without liver damage. Urinary excretion of 8-OHdG was not changed in chronic viral hepatitis.

CONCLUSIONS:

The results of the present study suggest that expression of oxidative stress-related molecules in leukocytes is upregulated in relation to serum aminotransferase levels.

• Authors: Toshiya Saeki, Miho Ichiba, Naotada Tanabe, Masaru Ueki, Kinya Okamoto, Yoshiko Matsunaga, Keiko Hosho, Takamasa Kanbe, Hiroyuki Tsuchiya, Akihiro Kurimasa, Sadako Yamada, Yasuaki Hirooka, Ichiro Hisatome, Yukihiro Kishimoto, Takeaki Suou, Yoshikazu Murawaki, Hironaka Kawasaki, Junji Yodoi, Goshi Shiota
• Journal: Liver international : official journal of the International Association for the Study of the Liver (Liver Int) Vol. 26 Issue 2 Pg. 157-65 (Mar 2006) ISSN: 1478-3223 [Print] United States
• PMID: 16448453 (Publication Type: Journal Article)

Chemical References

• Aldehydes
• Enzymes
• 4-hydroxy-2-hexenal
• Thioredoxins
• 8-Hydroxy-2'-Deoxyguanosine
• Aspartate Aminotransferases
• Alanine Transaminase
• Deoxyguanosine
• 4-hydroxy-2-nonenal

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Aged
• Alanine Transaminase (blood)
• Aldehydes (metabolism)
• Aspartate Aminotransferases (blood)
• Blotting, Western
• Deoxyguanosine (analogs & derivatives, urine)
• Enzyme-Linked Immunosorbent Assay
• Enzymes
• Female
• Hepatitis, Viral, Human (blood, pathology, urine)
• Humans
• Leukocytes (metabolism, pathology)
• Lipid Peroxidation
• Liver (metabolism, pathology)
• Male
• Middle Aged
• Oxidative Stress
• Thioredoxins (metabolism)
• Up-Regulation