Acute liver damage induced by 2-nitropropane in rats: effect of diphenyl diselenide on antioxidant defenses.

Abstract

The effect of post-treatment with diphenyl diselenide on liver damage induced by 2-nitropropane (2-NP) was examined in male rats. Rats were pre-treated with a single dose of 2-NP (100 mg/kg body weight dissolved in canola oil). Afterward, the animals were post-treated with a dose of diphenyl diselenide (10, 50 or 100 micromol/kg). The parameters that indicate tissue damage such as liver histopathology, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), urea and creatinine were determined. Since the liver damage induced by 2-NP is related to oxidative damage, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT) and ascorbic acid level were also evaluated. Diphenyl diselenide (50 and 100 micromol/kg) effectively restored the increase of ALT and AST activities and urea level when compared to the 2-NP group. At the higher dose, diphenyl diselenide decreased GGT activity. Treatment with diphenyl diselenide, at all doses, effectively ameliorated the increase of hepatic and renal lipid peroxidation when compared to 2-NP group. 2-NP reduced CAT activity and neither alter SOD activity nor ascorbic acid level. This study points out the involvement of CAT activity in 2-NP-induced acute liver damage and suggests that the post-treatment with diphenyl diselenide was effective in restoring the hepatic damage induced by 2-NP.

• Authors: Lysandro P Borges, Cristina Wayne Nogueira, Rodrigo B Panatieri, João Batista Teixeira Rocha, Gilson Zeni
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 160 Issue 2 Pg. 99-107 (Mar 25 2006) ISSN: 0009-2797 [Print] Ireland
• PMID: 16445897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Benzene Derivatives
• Enzymes
• Free Radical Scavengers
• Nitroparaffins
• Organoselenium Compounds
• Reactive Oxygen Species
• Solvents
• diphenyldiselenide
• 2-nitropropane
• Propane

Topics

• Acute Disease
• Animals
• Antioxidants (pharmacology)
• Benzene Derivatives (pharmacology)
• Chemical and Drug Induced Liver Injury (metabolism, pathology, prevention & control)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Interactions
• Enzymes (metabolism)
• Free Radical Scavengers
• Kidney (drug effects, metabolism)
• Lipid Peroxidation (drug effects)
• Liver (drug effects, metabolism, pathology)
• Liver Function Tests
• Male
• Nitroparaffins (toxicity)
• Organoselenium Compounds (pharmacology)
• Propane (analogs & derivatives, toxicity)
• Rats
• Rats, Wistar
• Reactive Oxygen Species (metabolism)
• Solvents (toxicity)