Abstract |
Currently cis-diamminedichloroplatinum(II) (cis-DDP) is one of the most commonly applied compounds in chemotherapy of many types of cancer. However, a drawback is that its effectiveness presents with many side effects. Therefore, human normal lymphocytes were chosen as a model system to study cis-bis(3-aminoflavone)dichloroplatinum(II) (the cis-Pt(II) complex of 3- aminoflavone) in comparison with cis-DDP. We examined the effect of both tested compounds on cell viability and induction of apoptosis and necrosis. Trypan blue and acridine orange/ ethidium bromide staining were carried out, as well as quantitative analysis of the apoptotic signal of P53 and BAX induction caused by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP. cis-DDP induced a decrease of cell viability and led to a higher increase in necrosis and apoptosis than did the cis-Pt(II) complex of 3-aminoflavone. Moreover, at the molecular level cis-DDP increased P53 and BAX expression in comparison with the other tested compound. The cis-Pt(II) complex of 3-aminoflavone showed a weaker genotoxic effect in normal lymphocytes in comparison with cis-DDP, which was a stronger inducer of apoptosis and necrosis.
|
Authors | Beata Kosmider, Izabela Zawlik, Pawel P Liberski, Regina Osiecka, Elzbieta Zyner, Justyn Ochocki, Jacek Bartkowiak |
Journal | Mutation research
(Mutat Res)
Vol. 604
Issue 1-2
Pg. 28-35
(Apr 30 2006)
ISSN: 0027-5107 [Print] Netherlands |
PMID | 16443387
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- 3-aminoflavone
- DNA Primers
- Flavonoids
- RNA, Messenger
- bcl-2-Associated X Protein
- Cisplatin
|
Topics |
- Apoptosis
(drug effects)
- Base Sequence
- Cell Culture Techniques
- Cell Survival
(drug effects)
- Cisplatin
(toxicity)
- DNA Primers
- Flavonoids
(toxicity)
- Gene Expression Regulation
(drug effects)
- Genes, p53
(drug effects)
- Humans
- Lymphocytes
(cytology, drug effects, physiology)
- Necrosis
- RNA, Messenger
(genetics)
- bcl-2-Associated X Protein
(drug effects, genetics)
|