Endothelin-3 production by human rhabdomyosarcoma: a possible new marker with a paracrine role.

Abstract

Several autocrine and paracrine growth factor circuits have been found in human rhabdomyosarcoma cells. In this study we show that endothelin-3 (ET-3), a vasoactive peptide, is produced by human rhabdomyosarcoma cell lines, whereas it is not expressed by human sarcoma cell lines of non-muscle origin. We did not find evidence of a significant autocrine loop; nevertheless ET-3 produced by rhabdomyosarcoma cells can act as a paracrine factor, since it promotes migration of endothelial cells. Moreover ET-3 is present in plasma of mice bearing xenografts of human rhabdomyosarcoma cells, and may be potential new marker of the human rhabdomyosarcoma to be studied further.

• Authors: Arianna Palladini, Annalisa Astolfi, Stefania Croci, Carla De Giovanni, Giordano Nicoletti, Angelo Rosolen, Francesca Sartori, Pier-Luigi Lollini, Lorena Landuzzi, Patrizia Nanni
• Journal: European journal of cancer (Oxford, England : 1990) (Eur J Cancer) Vol. 42 Issue 5 Pg. 680-7 (Mar 2006) ISSN: 0959-8049 [Print] England
• PMID: 16439111 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Endothelin-3
• Oligopeptides
• Piperidines
• Receptors, Endothelin
• BQ 788

Topics

• Biomarkers, Tumor (biosynthesis)
• Cell Line, Tumor
• Dose-Response Relationship, Drug
• Endothelin-3 (biosynthesis)
• Humans
• Immunoassay (methods)
• Oligopeptides (pharmacology)
• Osteosarcoma (metabolism)
• Paracrine Communication
• Piperidines (pharmacology)
• Receptors, Endothelin (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Rhabdomyosarcoma (metabolism)
• Sarcoma, Ewing (metabolism)