Abstract |
DNA immunization in conjunction with antiretroviral therapy was evaluated in SIV-infected rhesus macaques treated with [R]-9-[2-phosphonylmethoxypropyl] adenine (PMPA). Macaques were immunized monthly with DNA vaccines expressing either SIV gag/tat or SIV gag/tat and 19 CD8+ T cell epitopes during 7 months of therapy. Half the animals from each group were additionally immunized before infection. Only 60% of the animals (4 controls, 20 vaccinated) responded to PMPA (ART responders). All 4 ART responder controls demonstrated viral rebound or CD4 decline after PMPA was withdrawn. In contrast, 17 of 20 vaccinated ART responders contained viral rebound for over 7 months after PMPA was withdrawn. Viral control correlated with stable CD4 counts, higher lymphoproliferation and an increase in the magnitude and breadth of the CD8+ T cell response. Immunizing before infection or with multi- epitopes enhanced these effects. These results demonstrate that DNA immunization during antiretroviral therapy may be an effective strategy to treat HIV infection.
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Authors | Deborah Heydenburg Fuller, Premeela A Rajakumar, Mary S Wu, Christopher W McMahon, Tim Shipley, James T Fuller, Afrouz Bazmi, Anita M Trichel, Todd M Allen, Bianca Mothe, Joel R Haynes, David I Watkins, Michael Murphey-Corb |
Journal | Virology
(Virology)
Vol. 348
Issue 1
Pg. 200-15
(Apr 25 2006)
ISSN: 0042-6822 [Print] United States |
PMID | 16439000
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Anti-HIV Agents
- Epitopes, T-Lymphocyte
- Gene Products, gag
- Gene Products, tat
- Organophosphonates
- RNA, Viral
- Vaccines, DNA
- Tenofovir
- Adenine
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Topics |
- Adenine
(administration & dosage, analogs & derivatives, therapeutic use)
- Animals
- Anti-HIV Agents
(administration & dosage, therapeutic use)
- CD4 Lymphocyte Count
- CD8-Positive T-Lymphocytes
(immunology)
- Cell Proliferation
- Disease Progression
- Epitopes, T-Lymphocyte
(genetics, immunology)
- Gene Products, gag
(genetics, immunology)
- Gene Products, tat
(genetics, immunology)
- Immunotherapy, Active
(methods)
- Macaca mulatta
- Organophosphonates
(administration & dosage, therapeutic use)
- RNA, Viral
(blood)
- Simian Acquired Immunodeficiency Syndrome
(drug therapy, immunology)
- Simian Immunodeficiency Virus
(drug effects, immunology)
- Statistics as Topic
- Tenofovir
- Vaccines, DNA
(administration & dosage, immunology)
- Viremia
(prevention & control)
- Withholding Treatment
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