Abstract | BACKGROUND: Fluorescence imaging is an attractive diagnostic technique for skin tumour demarcation with potential to move to clinical use. Bispectral fluorescence imaging combines skin autofluorescence with delta-aminolaevulinic acid-induced fluorescence. To evaluate the technique, fluorescence data must be compared with the histopathological extent of the tumour, which is the purpose of the current study. OBJECTIVES: To investigate the agreement between bispectral fluorescence images and the histopathological tumour boundary of ill-defined basal cell carcinomas (BCCs). After fluorescence imaging the tumours were removed using Mohs micrographic surgery (MMS) to obtain histopathological maps of the tumour boundaries. METHODS: Twelve patients with aggressive BCC of mean diameter 16 mm (range 5-32) in the face were included in the study. The patients were subjected to bispectral fluorescence imaging within the 2 months prior to MMS. The fluorescence images and histopathological maps were aligned using image warping. RESULTS: Five patients (42%) showed good agreement with the histopathological mapping and the remaining seven patients (58%) showed partial agreement. Bispectral investigation combining autofluorescence with protoporphyrin IX ( PpIX) fluorescence generally yielded better agreement with the histopathological boundaries of the tumours compared with using only the PpIX fluorescence. CONCLUSIONS: In this preliminary study the fluorescence has been compared with the histopathological tumour boundaries. The result implies that the technique can be applied as a useful tool for indicating tumour boundary of aggressive BCCs. Further refinement is needed to be able to indicate the exact tumour border.
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Authors | B Stenquist, M B Ericson, C Strandeberg, L Mölne, A Rosén, O Larkö, A M Wennberg |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 154
Issue 2
Pg. 305-9
(Feb 2006)
ISSN: 0007-0963 [Print] England |
PMID | 16433801
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Photosensitizing Agents
- Aminolevulinic Acid
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Aminolevulinic Acid
- Carcinoma, Basal Cell
(pathology, surgery)
- Facial Neoplasms
(pathology, surgery)
- Female
- Fluorescence
- Humans
- Image Processing, Computer-Assisted
(methods)
- Male
- Middle Aged
- Mohs Surgery
- Photosensitizing Agents
- Skin Neoplasms
(pathology, surgery)
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