Serological immune response to cancer testis antigens in patients with pancreatic cancer.

Abstract

Serological screening approaches have allowed for the identification of a large number of potentially relevant tumor antigens in cancer patients. Within this group, cancer testis antigens represent promising targets for cancer immunotherapy, since they are widely expressed in a variety of human cancer entities. In pancreatic cancer, however, there are only few data available about the expression pattern and serological response to cancer testis antigens and other serological-defined tumor antigens. Therefore, we investigated the IgG antibody response against 11 cancer testis antigens (SCP-1, GAGE, LAGE-1a,-1b, CT-7, NY-ESO-1, SSX-1-5) recombinantly expressed on yeast surface (RAYS) in patients with pancreatic cancer (n = 96), chronic pancreatitis (n = 18) and healthy donors (n = 48). We found in 14% of all patients antibody responses to SCP-1, but not to other cancer testis antigens (GAGE, LAGE-1a,-1b, CT-7, NY-ESO-1, SSX-1-5). Antibody response correlated with the expression of SCP-1 in the primary tumor of the respective patient as shown by RT-PCR, immunohistochemistry and Western blot. In contrast, no serological response to cancer testis antigens was observed in healthy donors. The humoral immune response against SCP-1 was associated with the size of tumor, but not with other clinico-pathological parameters such as histology, stage, presence of lymph node metastases, grading, age, gender or gemcitabine treatment. In conclusion, antibody response to cancer testis antigen SCP-1 is found in a proportion of pancreatic carcinoma patients. These results indicate that identification of additional tumor antigens by serological screening of tumor cDNA expression libraries by RAYS is a promising goal in pancreatic cancer.

• Authors: Andreas Wadle, Boris Kubuschok, Jochen Imig, Beate Wuellner, Christine Wittig, Carsten Zwick, Axel Mischo, Kristin Waetzig, Bernd F M Romeike, Werner Lindemann, Martin Schilling, Michael Pfreundschuh, Christoph Renner
• Journal: International journal of cancer (Int J Cancer) Vol. 119 Issue 1 Pg. 117-25 (Jul 01 2006) ISSN: 0020-7136 [Print] United States
• PMID: 16432832 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• CTAG1B protein, human
• Membrane Proteins
• Nuclear Proteins
• CTDSP1 protein, human
• Phosphoprotein Phosphatases

Topics

• Aged
• Antigens, Neoplasm (immunology)
• Biomarkers, Tumor (immunology)
• Blotting, Western
• Cell Line, Tumor
• Female
• Flow Cytometry
• Fluorescent Antibody Technique
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Male
• Membrane Proteins (immunology)
• Middle Aged
• Nuclear Proteins
• Pancreatic Neoplasms (immunology)
• Phosphoprotein Phosphatases (immunology)
• Reverse Transcriptase Polymerase Chain Reaction
• Testis