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Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK.

AbstractOBJECTIVES:
Cytotoxic methacrylate monomers have been identified in aqueous extracts of freshly cured compomers. Some of these compounds, including HEMA and TEGDMA, induce apoptosis and necrosis in vitro. The aim of the present study was to elucidate possible signaling pathways involved in apoptosis following exposure to HEMA or TEGDMA in a salivary gland cell line.
METHODS:
The cells were exposed to various concentrations of HEMA or TEGDMA. ROS formation was determined by dichlorofluorescein assay. Phosphorylated MAP-kinases ERK1/2, p38 and JNK, as well as specific caspases were identified by Western blotting. Apoptosis was assayed by fluorescence microscopy.
RESULTS:
HEMA or TEGDMA exposure resulted in ROS formation and concentration-dependent apoptosis as well as phosphorylation of ERK. Phosphorylation of JNK and p38 was induced by HEMA. Selective inhibitors of ERK and JNK modified the apoptotic response after HEMA and TEGDMA exposure, whereas p38 inhibition modified the apoptotic response only after HEMA exposure. Vitamin C reduced HEMA-induced apoptosis.
SIGNIFICANCE:
ROS formation and differential MAP kinase activation appear to be involved in the apoptotic response following exposure to HEMA and TEGDMA.
AuthorsJan T Samuelsen, Jon E Dahl, Stig Karlsson, Else Morisbak, Rune Becher
JournalDental materials : official publication of the Academy of Dental Materials (Dent Mater) Vol. 23 Issue 1 Pg. 34-9 (Jan 2007) ISSN: 0109-5641 [Print] England
PMID16430953 (Publication Type: Journal Article)
Chemical References
  • Antioxidants
  • Dental Materials
  • Flavonoids
  • Fluorescent Dyes
  • Methacrylates
  • Polymethacrylic Acids
  • Reactive Oxygen Species
  • triethylene glycol dimethacrylate
  • Polyethylene Glycols
  • hydroxyethyl methacrylate
  • Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 4
  • Caspases
  • Ascorbic Acid
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
Topics
  • Animals
  • Antioxidants (pharmacology)
  • Apoptosis (drug effects)
  • Ascorbic Acid (pharmacology)
  • Blotting, Western
  • Caspases (metabolism)
  • Cell Line
  • Dental Materials (pharmacology)
  • Enzyme Activation (drug effects)
  • Flavonoids (pharmacology)
  • Fluorescent Dyes
  • MAP Kinase Kinase 4 (antagonists & inhibitors, metabolism)
  • Methacrylates (pharmacology)
  • Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)
  • Polyethylene Glycols (pharmacology)
  • Polymethacrylic Acids (pharmacology)
  • Rats
  • Reactive Oxygen Species (metabolism)
  • Signal Transduction (drug effects)
  • Time Factors
  • p38 Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)

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