A pseudotype vesicular stomatitis virus containing Hantaan virus envelope glycoproteins G1 and G2 as an alternative to hantavirus vaccine in mice.

We examined whether a vesicular stomatitis virus (VSV) pseudotype bearing the hantavirus envelope glycoproteins (GPs) G1 and G2 (VSVdeltaG*HTN) could be used as a safe and effective alternative to native hantavirus. Mice were immunized with purified particles of VSVdeltaG*HTN. After the second immunization, all mice produced anti-GP antibody as detected in ELISA and a neutralization test. After the third immunization, the mice were challenged with Hantaan virus. Neither anti-NP antibody production nor Hantaan virus-specific CD8 T-cell reactions were detected in these mice. The present study demonstrated the potential of using a pseudotype VSV system as a tool for developing a hantavirus vaccine.
AuthorsByoung-Hee Lee, Kumiko Yoshimatsu, Koichi Araki, Megumi Okumura, Ichiro Nakamura, Jiro Arikawa
JournalVaccine (Vaccine) Vol. 24 Issue 15 Pg. 2928-34 (Apr 5 2006) ISSN: 0264-410X [Print] Netherlands
PMID16426712 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Vaccines, Synthetic
  • Viral Envelope Proteins
  • Viral Vaccines
  • glycoprotein G1, Hantavirus
  • glycoprotein G2, Hantavirus
  • Interferon-gamma
  • Animals
  • Antibodies, Viral (blood)
  • CD8-Positive T-Lymphocytes (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Hantavirus (genetics, immunology)
  • Hantavirus Infections (prevention & control)
  • Interferon-gamma (biosynthesis)
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Vaccines, Synthetic (genetics, immunology)
  • Vesicular stomatitis Indiana virus (genetics, immunology)
  • Viral Envelope Proteins (genetics, immunology)
  • Viral Vaccines (genetics, immunology)

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