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Cytokine and Toll-like receptor mRNAs in the nasal-associated lymphoid tissues of cattle during foot-and-mouth disease virus infection.

Abstract
The pharyngeal region is known to play an important role in foot-and-mouth disease virus (FMDV) infection in relation to acute disease and viral persistence. In this study, the local mucosal immune response in nasal-associated lymphoid tissue (NALT) of cattle infected with FMDV (strain O UKG 34/2001) was examined. Quantitative "real-time" RT-PCR assays were used to measure mRNA expression of cytokines (IFN-alpha, beta and gamma, IL-2, IL-1alpha and TNF-alpha) and Toll-like receptors (TLR)-3 and -4. NALTs from dorsal soft palate were collected from cattle at 7 days post-infection (dpi) and from carriers and non-carriers at 64 dpi. Expression of IFN-alpha mRNA was significantly greater in NALT during acute disease than in uninfected animals. Increased expression of IFN-gamma and IL-1alpha mRNA was also observed but was much lower than IFN-alpha expression. There was a slight increase in mRNA expression of TNF-alpha and IL-2. During persistence, TNF-alpha mRNA expression in carrier cattle was much higher than in non-carrier cattle. Expression of TLR-4 in NALT during the acute stage of infection was greater than in uninfected animals. Carrier and non-carrier cattle did not differ in respect of expression of TLR-3 and -4 mRNA in NALT.
AuthorsZ Zhang, J B Bashiruddin, C Doel, J Horsington, S Durand, S Alexandersen
JournalJournal of comparative pathology (J Comp Pathol) Vol. 134 Issue 1 Pg. 56-62 (Jan 2006) ISSN: 0021-9975 [Print] England
PMID16423571 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Messenger
  • Receptors, Cytokine
  • Receptors, Interleukin
  • Receptors, Tumor Necrosis Factor
  • Toll-Like Receptor 3
  • Toll-Like Receptor 4
  • Toll-Like Receptors
Topics
  • Animals
  • Cattle
  • Cattle Diseases (immunology)
  • Foot-and-Mouth Disease (immunology)
  • Lymphoid Tissue (immunology, metabolism)
  • Nasal Mucosa (metabolism)
  • RNA, Messenger (metabolism)
  • Receptors, Cytokine (metabolism)
  • Receptors, Interleukin (metabolism)
  • Receptors, Tumor Necrosis Factor
  • Reverse Transcriptase Polymerase Chain Reaction
  • Toll-Like Receptor 3 (metabolism)
  • Toll-Like Receptor 4 (metabolism)
  • Toll-Like Receptors (metabolism)

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