[Effects of ginsenoside Rh2(GS-Rh2) on cell cycle of Eca-109 esophageal carcinoma cell line].

Abstract

OBJECTIVE:

To investigate the effects of ginsenoside Rh2 (GS-Rh2) on growth inhibition and cell cycle of Eca-109 esophageal carcinoma cell line in culture.

METHOD:

The effects of GS-Rh2 on cell growth inhibition was detected by MTT assay. Cell cycle was analyzed by flow cytometry (FCM). Cell morphology was observed by a light microscope after HE staining. The protein expression of cell cycle components (cyclinE, CDK2, p21WAF1) were examined by immunocytochemistry and Western blot. The mRNA expression were examined by semiquantitative RT-PCR.

RESULT:

GS-Rh2 inhibited the proliferation of Eca-109 cells in dose and time-dependent manners. The inhibition rate was about 50% after 1-day treatment with 20 microg x mL(-1) GS-Rh2 x 20 microg x mL(-1) GS-Rh2 induced the mature differentiation and morphological reversion. With increasing dose of GS-Rh2 treatment, the cell number of G0/G1 phase was increased, whereas it decreased at S and G2/M phase. There was significant difference between 10, 20 microg x mL(-1) GS-Rh2 groups and the corresponding group without GS-Rh2 treatement. After treating cells by 20 microg x mL(-1) GS-Rh2 for 1, 2, 3 days individually, the protein and mRNA expression of both cyclinE and CDK2 reduced, while the expression of p21WAF1 enhanced gradually.

CONCLUSION:

GS-Rh2 could arrest Eca-109 cells at G0/G1 phase and induce cell differentiation tending to normal. Furthermore, GS-Rh2 had an effect on expression of cell cycle components (cyclinE, CDK2 and p21WAF1) to inhibit Eca-109 cell proliferation.

• Authors: Li Li, Feng-ying Qi, Jun-ru Liu, Lian-fu Zuo
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 30 Issue 20 Pg. 1617-21 (Oct 2005) ISSN: 1001-5302 [Print] China
• PMID: 16422547 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CDKN1A protein, human
• Cyclin E
• Cyclin-Dependent Kinase Inhibitor p21
• Drugs, Chinese Herbal
• Ginsenosides
• RNA, Messenger
• ginsenoside Rh2
• CDK2 protein, human
• Cyclin-Dependent Kinase 2

Topics

• Carcinoma, Squamous Cell (pathology)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cyclin E (biosynthesis, genetics)
• Cyclin-Dependent Kinase 2 (biosynthesis, genetics)
• Cyclin-Dependent Kinase Inhibitor p21 (biosynthesis, genetics)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (administration & dosage, isolation & purification, pharmacology)
• Esophageal Neoplasms (metabolism, pathology)
• Ginsenosides (administration & dosage, isolation & purification, pharmacology)
• Humans
• Panax (chemistry)
• Plants, Medicinal (chemistry)
• RNA, Messenger (biosynthesis, genetics)
• Time Factors