A study of the relationship between expression level of TRF1 protein and telomerase activity in human acute leukemia.

Abstract

OBJECTIVE:

To study the expression level of TRF1 (telomeric repeat binding factor 1) protein in human acute leukemia and relationship between expression level of TRF1 protein and telomerase.

METHODS:

A quantitative Western-Blot technique was developed using anti-TRF1(33-277) monoclonal antibody and GST-TRF1 purity protein as a standard to further determine the expression level of TRF1 protein in total proteins extracted from clinical specimens.

RESULTS:

Bone marrow tissues of 20 acute leukemia patients were studied, 11 healthy donors' bone marrows were taken as a control. The expression level of TRF1 protein was significantly higher (P<0.01) in normal bone marrow ((2.217+/-0.462) microg/microl) than that of acute leukemia patients ((0.754+/-0.343) microg/microl). But there was no remarkable difference between ALL and ANLL patients ((0.618+/-0.285) microg/microl vs (0.845+/-0.359) microg/microl, P>0.05). After chemotherapy, TRF1 expression level of patients with complete remission elevated ((0.772+/-0.307) microg/microl vs (1.683+/-0.344) microg/microl, P<0.01), but lower than that of normal ((2.217+/-0.462) microg/microl, P<0.01). There was no significantly difference after chemotherapy ((0.726+/-0.411) microg/microl vs (0.895+/-0.339) microg/microl, P>0.05). TRF1 expression level of patients with complete remission is higher than that of patients without complete remission ((1.683+/-0.344) microg/microl vs (0.895+/-0.339) microg/microl, P<0.01). All samples were determined for telomerase activity. It was confirmed that the activity of telomerase in normal bone marrow was lower than that of acute leukemia patients ((0.125+/-0.078) microg/microl vs (0.765+/-0.284) microg/microl, P<0.01). There was no significant difference of expression level of TRF1 protein between ALL and ANLL patients ((0.897+/-0.290) microg/microl vs (0.677+/-0.268) microg/microl, P>0.05). After chemotherapy, telomerase activity of patients with complete remission decreased ((0.393+/-0.125) microg/microl), but was still higher than that of normal ((0.125+/-0.078) microg/microl, P<0.01).

CONCLUSION:

The expression level of TRF1 protein has correlativity to the activity of telomerase (P<0.001).

• Authors: Ji-min Shi, He Huang, Qiao-fang Chen, Mao-fang Lin
• Journal: Journal of Zhejiang University. Science. B (J Zhejiang Univ Sci B) Vol. 7 Issue 2 Pg. 154-8 (Feb 2006) ISSN: 1673-1581 [Print] China
• PMID: 16421973 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Telomeric Repeat Binding Protein 1
• Telomerase

Topics

• Adolescent
• Adult
• Aged
• Antibodies, Monoclonal (chemistry)
• Blotting, Western
• Bone Marrow Cells (metabolism)
• Child
• Dose-Response Relationship, Drug
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Leukemia, Myeloid, Acute (enzymology)
• Male
• Middle Aged
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (enzymology)
• Remission Induction
• Telomerase (biosynthesis, metabolism)
• Telomeric Repeat Binding Protein 1 (biosynthesis)
• Treatment Outcome