1-hydroxyPGE reduces infarction volume in mouse transient cerebral ischemia.

Abstract

Differential neurological outcomes due to prostaglandin E2 activating G-protein-coupled prostaglandin E (EP) receptors have been observed. Here, we investigated the action of the EP4/EP3 agonist 1-hydroxyPGE1 (1-OHPGE1) in modulating transient ischemic brain damage. C57BL/6 mice were pretreated 50 min before transient occlusion of the middle cerebral artery with an intraventricular injection of 1-OHPGE1 (0.1, 0.2, 2.0 nmol/0.2 microL). Brain damage 4 days after reperfusion, as estimated by infarct volume, was significantly reduced by more than 19% with 1-OHPGE1 in the two higher-dose groups (P < 0.05). To further address whether protection also was extended to neurons, primary mouse cultured neuronal cells were exposed to N-methyl-D-aspartate. Co-treatment with 1-OHPGE1 resulted in significant neuroprotection (P < 0.05). To better understand potential mechanisms of action and to test whether changes in cyclic adenosine monophosphate (cAMP) levels and downstream signaling would be neuroprotective, we measured cAMP levels in primary neuronal cells. Brief exposure to 1-OHPGE1 increased cAMP levels more than twofold and increased the phosphorylation of extracellular-regulated kinases at positions Thr-202/Tyr-204. In a separate cohort of animals, 1-OHPGE1 at all doses tested produced no significant effect on the physiological parameters of core body temperature, mean arterial pressure and relative cerebral blood flow observed following drug treatment. Together, these results suggest that modulation of PGE2 receptors that increase cAMP levels and activate extracellular-regulated kinases 1/2 caused by treatment with 1-OHPGE1 can be protective against neuronal injury induced by focal ischemia.

• Authors: Muzamil Ahmad, Sofiyan Saleem, Hean Zhuang, Abdullah Shafique Ahmad, Valentina Echeverria, Adam Sapirstein, Sylvain Doré
• Journal: The European journal of neuroscience (Eur J Neurosci) Vol. 23 Issue 1 Pg. 35-42 (Jan 2006) ISSN: 0953-816X [Print] France
• PMID: 16420413 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Neuroprotective Agents
• Ptger3 protein, mouse
• Receptors, Prostaglandin E
• Receptors, Prostaglandin E, EP3 Subtype
• Cyclic AMP
• Alprostadil

Topics

• Alprostadil (therapeutic use)
• Analysis of Variance
• Animals
• Blood Gas Analysis (methods)
• Blood Pressure (drug effects)
• Blotting, Western (methods)
• Body Temperature (drug effects)
• Brain Infarction (etiology, prevention & control)
• Cells, Cultured
• Cyclic AMP (metabolism)
• Disease Models, Animal
• Embryo, Mammalian
• Ischemic Attack, Transient (complications, drug therapy, pathology)
• Male
• Mice
• Mice, Inbred C57BL
• Neurologic Examination
• Neuroprotective Agents (therapeutic use)
• Receptors, Prostaglandin E (antagonists & inhibitors)
• Receptors, Prostaglandin E, EP3 Subtype