Abstract | OBJECTIVE: MATERIALS AND METHODS: Three pairs of heterozygote mto mice, SWR/J-Clcn1(adr-mto/+) and ten Wistar rats were used. We performed intracellular recordings of myotonia from mto mice and the drug effects on insertion myotonia were recorded from the hemidiaphragm preparations of mto mice with different concentrations of DHEAS. Isometric twitch tension was recorded from rat hemidiaphragm preparations in Tyrode's solution and the effect of DHEAS on the muscle twitch tension was measured at different concentrations of DHEAS from 100 mg/l to 300 mg/l. The effect of mexiletine on ITT was also measured. RESULTS: In mto mice insertion myotonia was recorded as soon as the microelectrode was inserted in the muscle cells. When DHEAS was added to Tyrode's solution, insertion myotonia was suppressed. DHEAS decreased ITT up to 70% of the original value, though mexiletine decreased ITT to 30% of the original value. Therefore, the decrement of the muscle strength in DHEAS solution is much smaller than that of mexiletine. CONCLUSION:
|
Authors | Hiroshi Nakazora, Teruyuki Kurihara |
Journal | Internal medicine (Tokyo, Japan)
(Intern Med)
Vol. 44
Issue 12
Pg. 1247-51
(Dec 2005)
ISSN: 0918-2918 [Print] Japan |
PMID | 16415544
(Publication Type: Comparative Study, Journal Article)
|
Chemical References |
- Dehydroepiandrosterone Sulfate
|
Topics |
- Action Potentials
(drug effects, physiology)
- Animals
- Dehydroepiandrosterone Sulfate
(therapeutic use)
- Disease Models, Animal
- Electrophysiology
(methods)
- Isometric Contraction
(drug effects)
- Mice
- Muscle, Skeletal
(drug effects, pathology, physiopathology)
- Myotonia
(drug therapy, metabolism, pathology)
- Rats
- Rats, Wistar
|