Expression of matrix metalloproteinase-9 and -2 in pediatric chronic otitis media with effusion.

Abstract

OBJECTIVE:

To evaluate the role of MMP-9 and MMP-2 in pediatric patients with middle ear effusion (MEE) and determine the pathogenesis of otitis media with effusion (OME) and allergy.

METHODS:

The MEE samples were collected from 25 patients with allergy and 20 patients without allergy as a control. The levels of MMP-9 and MMP-2 were measured using a commercially available enzyme-linked immunosorbent assay (ELISA). The activity of MMP-9 and MMP-2 were measured by gelatin-zymography. The Mann-Whitney U-test was used for comparisons between the allergy positive and control groups.

RESULTS:

The level of MMP-9 was significantly elevated in MEE from the allergy positive group compared to controls. The amount of MMP-9 activity significantly increased in the allergy positive group compared to controls.

CONCLUSION:

MMP-9 and MMP-2 are mediators of inflammation in the OME; in addition, MMP-9 may play a significant role in the pathophysiology of OME with allergy.

• Authors: Chul-Ho Jang, Sung-Heui Shin, Hyong-Ho Cho, Seok-Jin Moon, Yong-Bum Cho
• Journal: International journal of pediatric otorhinolaryngology (Int J Pediatr Otorhinolaryngol) Vol. 70 Issue 7 Pg. 1155-8 (Jul 2006) ISSN: 0165-5876 [Print] Ireland
• PMID: 16413616 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Inflammation Mediators
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Child
• Child, Preschool
• Chronic Disease
• Enzyme-Linked Immunosorbent Assay
• Humans
• Hypersensitivity (metabolism, physiopathology)
• Inflammation Mediators (metabolism)
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Otitis Media with Effusion (metabolism, physiopathology)
• Respiratory Mucosa (metabolism, physiopathology)