Preventive strategy for Candida gut translocation during ischemia-reperfusion injury supervening on protein-calorie malnutrition.

Abstract

OBJECTIVE:

The aim of the present study was to test the hypothesis that protein-calorie malnutrition aggravates the gut translocation of Candida albicans triggered by mesenteric ischemia-reperfusion (IR) injury in an experimental model while testing a natural product containing the antifungal anethole/polygodial mixture (Kolorex).

METHODS:

MFI strain white mice (n = 90) were randomly allocated to a 4-week dietary regimen: (1) standard pellet diet containing 25% casein; (2) low-protein (2.5%) casein diet; (3) as group 2 plus oral supplementation with 20 microL of a 5% solution of Kolorex during the last 4 days. Twenty rats from each of these groups (termed 1a, 2a and 3a) were orally inoculated with Candida suspension 6 h prior to mesenteric IR injury. Animals of each group but without Candida inoculation (termed 1b, 2b and 3b) served as control. A colon permeability study was carried out as well. Rats were killed prior to the IR injury and 3 h afterwards. Control rats were killed at the same time.

RESULTS:

Over 60% of the mesenteric lymph nodes and 30% of kidney samples were positive for C. albicans in the low-protein-fed rats after IR injury. Kolorex significantly decreased that rate of positivity and also significantly reduced the concentration of C. albicans per gram of each positive tissue sample examined. Protein-calorie malnourished animals showed a statistically significant increase in colon permeability and this phenomenon further increased after IR injury. The groups of rats treated with Kolorex compound showed a partial, although significant, improvement of this parameter.

CONCLUSIONS:

These results suggest that Kolorex might exert a competitive effect against with C. albicans colonization. The present study represents the first experimental in vivo investigation of the anethole/polygodial-containing compound under the specific conditions of calorie-protein malnutrition and the results have potential clinical interest.

• Authors: F Marotta, R Barreto, S Kawakita, E Minelli, K Pavasuthipaisit, A Lorenzetti, M Nishiwaki, F Gelosa, E Fesce, R Okura
• Journal: Chinese journal of digestive diseases (Chin J Dig Dis) Vol. 7 Issue 1 Pg. 33-8 ( 2006) ISSN: 1443-9611 [Print] Australia
• PMID: 16412035 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Allylbenzene Derivatives
• Anisoles
• Antifungal Agents
• Sesquiterpenes
• polygodial
• anethole

Topics

• Allylbenzene Derivatives
• Animals
• Anisoles (administration & dosage)
• Antifungal Agents (administration & dosage)
• Bacterial Translocation (drug effects)
• Candida albicans (physiology)
• Candidiasis (etiology, prevention & control)
• Disease Models, Animal
• Drug Therapy, Combination
• Intestinal Diseases (etiology, prevention & control)
• Kidney (microbiology)
• Lymph Nodes (microbiology)
• Mesentery (microbiology)
• Protein-Energy Malnutrition (complications)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (complications)
• Sesquiterpenes (administration & dosage)