HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Tumour budding at the deepest invasive margin correlates with lymph node metastasis in submucosal colorectal cancer detected by anticytokeratin antibody CAM5.2.

Abstract
In the past few years, tumour budding at the invasive margin has been reported as a new risk factor for lymph node metastasis in advanced colorectal cancers, but it is sometimes difficult to detect tumour budding in submucosal colorectal cancer by haematoxylin and eosin staining. We immunohistochemically examined tumour budding at the deepest invasive margin of 56 surgically resected submucosal colorectal carcinomas using anticytokeratin antibody CAM5.2, furthermore checked by AE1/AE3, and determined the relation between tumour budding and clinicopathological factors. Moreover, we used the monoclonal antibody D2-40 for immunohistochemistry to detect lymphatic involvement. Tumour budding was detected in 42 cases (75.0%), and the budding-positive group showed a significantly higher rate of lymph node metastasis (including isolated tumour cells) (16/42 vs 0/14; P=0.004) than the budding-negative group. The sensitivity and negative predictive value of tumour budding alone for lymph node metastasis were superior to those of lymphatic invasion alone. Furthermore, the specificity and positive predictive value of the combination of either lymphatic invasion or tumour budding were superior to those of lymphatic invasion alone. Tumour budding detected immunohistochemically by using CAM5.2 is a newly found risk factor for lymph node metastasis and may help to avoid oversurgery in the future.
AuthorsS Kazama, T Watanabe, Y Ajioka, T Kanazawa, H Nagawa
JournalBritish journal of cancer (Br J Cancer) Vol. 94 Issue 2 Pg. 293-8 (Jan 30 2006) ISSN: 0007-0920 [Print] England
PMID16404429 (Publication Type: Journal Article)
Chemical References
  • Antibodies
  • Biomarkers
  • CAM 5.2 antigen
  • Keratins
Topics
  • Adenocarcinoma (pathology)
  • Aged
  • Antibodies
  • Biomarkers (metabolism)
  • Colorectal Neoplasms (pathology)
  • Female
  • Humans
  • Immunohistochemistry
  • Keratins (immunology, metabolism)
  • Lymphatic Metastasis (pathology)
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • Risk Factors
  • Sensitivity and Specificity

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: