Abstract |
In our previous study, we showed that 4,4'-dihydroxybiphenyl (44'-BP) reduced melanin content via the inhibition of tyrosinase. In the current study, we utilized 44'-BP treated B16 melanoma cells (B16 cells) to measure several key cellular parameters known to be involved in melanogenic activity. Included in these measurements were tyrosinase and microphthalmia transcription factor ( MITF) protein levels, cyclic AMP levels, protein kinase A (PKA) activation, and reduced glutathione (GSH) and oxidized glutathione ( GSSG) levels. Results showed that 44'-BP effectively suppressed the amounts of tyrosinase and MITF proteins, cAMP levels, and PKA activation. In addition, 44'-BP enhanced the GSH/ GSSG ratio. In conclusion, our data provide an evidence that 44'-BP suppressed several cellular key parameters in the melanogenic pathway by downregulating the cAMP-dependent PKA signaling pathway and decreasing MITF gene expression (implied from the reduced protein levels), which in turn suppressed tyrosinase. We propose that the antimelanogenic action of 44'-BP is likely carried out by a combined effect of its anti-oxidant property and its ability to enhance intracellular GSH levels.
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Authors | Jae Kyung No, You Jung Kim, Jun Sik Lee, Hae Young Chung |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 29
Issue 1
Pg. 14-6
(Jan 2006)
ISSN: 0918-6158 [Print] Japan |
PMID | 16394501
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biphenyl Compounds
- Free Radical Scavengers
- Melanins
- Microphthalmia-Associated Transcription Factor
- Cyclic AMP
- Monophenol Monooxygenase
- Cyclic AMP-Dependent Protein Kinases
- Glutathione
- 4,4'-dihydroxybiphenyl
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Topics |
- Biphenyl Compounds
(pharmacology)
- Blotting, Western
- Cell Line, Tumor
- Cyclic AMP
(metabolism)
- Cyclic AMP-Dependent Protein Kinases
(metabolism)
- Down-Regulation
(drug effects)
- Free Radical Scavengers
(pharmacology)
- Gene Expression Regulation
- Glutathione
(metabolism)
- Humans
- Melanins
(biosynthesis)
- Melanoma, Experimental
(metabolism)
- Microphthalmia-Associated Transcription Factor
(genetics, metabolism)
- Monophenol Monooxygenase
(metabolism)
- Oxidation-Reduction
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