FR177391, a new anti-hyperlipidemic agent from Serratia. IV. Target identification and validation by chemical genetic approaches.

Abstract

Natural products with distinct biological activities are very promising molecular probes to dissect the novel pathways of biology. FR177391, a product of bacteria, was obtained as a natural compound possessing anti-hyperlipidemic effects. FR177391 enhances differentiation of mouse 3T3-L1 fibroblasts to adipocytes and reduces the circulating levels of triglyceride in C57BL/KsJ-db/db mice, a obese non-insulin-dependent diabetes mellitus animal model, although its mechanism of actions remained to be unknown. We report here that the target protein for FR177391 was identified to be protein phosphatase 2A (PP2A) by employing the method of affinity chromatography. FR177391 potently inhibited PP2A activity at nano molar concentration, and shared its binding pocket with a phosphatase inhibitor, okadaic acid. In addition to the phenotypic alterations, the enhancement for phosphorylation of extracellular signal-regulated kinase (ERK) protein was observed in the FR177391-treated 3T3-L1 cells. These results suggest that prolonged activation of ERK protein due to inhibition of its dephosphorylation by PP2A plays an important role in adipocyte maturation and regulation of the blood revels of lipids.

• Authors: Makiko Yamaoka, Kentaro Sato, Motoo Kobayashi, Nobuya Nishio, Mitsuru Ohkubo, Takashi Fujii, Hidenori Nakajima
• Journal: The Journal of antibiotics (J Antibiot (Tokyo)) Vol. 58 Issue 10 Pg. 654-62 (Oct 2005) ISSN: 0021-8820 [Print] England
• PMID: 16392682 (Publication Type: Journal Article)

Chemical References

• Hypolipidemic Agents

Topics

• Adipogenesis (drug effects)
• Animals
• Hypolipidemic Agents (chemistry, pharmacology)
• Mice
• Serratia (chemistry, metabolism)