FR177391, a new anti-hyperlipidemic agent from Serratia. III. Microbial conversion of FR177391 and synthesis of FR177391 derivatives for its target protein screening by chemical genetic approaches.

Abstract

FR177391 produced by Serratia liquefaciens No. 1821 enhances differentiation of mouse 3T3-L1 fibroblasts to adipocytes and reduces the circulating levels of triglyceride in C57BL/KsJ-db/bd mice, an obese non-insulin-dependent diabetes mellitus animal model, although its mechanism of actions remained to be unknown. Its active derivative, 20-hydroxy FR177391, and its inactive derivative, 3-hydroxy FR177391 were produced by microbial conversion of FR177391, and biotin-labeled FR177391 was synthesized from 20-hydroxy FR177391 as an active affinity ligand to identify target molecules of FR177391 by chemical genetic approaches.

• Authors: Motoo Kobayashi, Kentaro Sato, Seiji Yoshimura, Makiko Yamaoka, Shigehiro Takase, Mitsuru Ohkubo, Takashi Fujii, Hidenori Nakajima
• Journal: The Journal of antibiotics (J Antibiot (Tokyo)) Vol. 58 Issue 10 Pg. 648-53 (Oct 2005) ISSN: 0021-8820 [Print] England
• PMID: 16392681 (Publication Type: Journal Article)

Chemical References

• Hypolipidemic Agents

Topics

• Hypolipidemic Agents (chemical synthesis, metabolism, pharmacology)
• Magnetic Resonance Spectroscopy
• Serratia (chemistry)