Tumor-promoting activity of p-hydroxybenzenediazonium is accelerated in Mg-deficient rats.

Abstract

Tumor-promoting activity caused by the short-term administration of p-hydroxybenzenediazonium (PDQ) has been assayed in rats fed on a Mg-deficient diet as a reference model versus rats fed on a standard diet as controls. For 5 weeks groups of 20 rats, fed either on the Mg-deficient or standard diet, were treated simultaneously with PDQ. A group of 10 Mg-deficient rats remained untreated. Topical application of PDQ was followed by the appearance of macroscopic alterations in the skin, which were more evident in the Mg-deficient rats. No deaths occurred during the treatment. After 5 weeks' PDQ treatment the rats were killed and histological analyses were made. Tissues from the skin, liver, heart, kidney, lung and thymus were screened by conventional staining methods. Both the PDQ-treated Mg-deficient and PDQ-treated control rats presented tissue lesions, although to a different extent. The untreated Mg-deficient rats showed no such lesions. Mg-deficient rats treated with PDQ developed significant incipient fibrosarcomas (p<0.05) and extended hyperplasia (p<0.001), particularly in the skin, accompanied by a significant increase in the thickness of collagen (mean value: 445.4+/-47.2microm, p<0.05) compared to the control PDQ-treated group (mean values: 258.7+/-36.4microm). The overall results provide objective proof of tumor-promoting activity after 5 weeks' treatment with PDQ. Such a fast response is interpreted as being linked to the increased vulnerability of the membrane caused by Mg deficiency, which would more readily facilitate the toxic activity of p-hydroxybenzenediazonium ions.

• Authors: Bartolome Quintero, Elena Planells, María del Carmen Cabeza, Javier Esquivias, María del Pilar Gutiérrez, Cristina Sánchez, Pilar Aranda, Antonio Zarzuelo, Juan Llopis
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 159 Issue 3 Pg. 186-95 (Feb 25 2006) ISSN: 0009-2797 [Print] Ireland
• PMID: 16387288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Diazonium Compounds
• p-hydroxybenzenediazonium

Topics

• Animals
• Body Weight (drug effects)
• Diazonium Compounds (chemistry, pharmacology)
• Magnesium Deficiency (blood, physiopathology)
• Male
• Neoplasms (chemically induced, pathology)
• Rats
• Rats, Wistar
• Spectrum Analysis