Abstract | OBJECTIVE: METHODS: We used a combination of in vitro binding assays and co-immunoprecipitation (IP) experiments to investigate whether TAK1 interacted with the type I (ALK1 or ALK5) or type II (TBRII) TGFbeta receptors. Interactions between endogenous proteins were tested using mouse myoblast and rat cardiomyocyte cells. RESULTS: Immunoprecipitation and in vitro binding assays show that TAK1 binds directly to TBRII. Precipitation of endogenous TAK1 protein in rat cardiomyocytes shows that, in addition to a direct association with TBRII, it also interacts indirectly with ALK5. CONCLUSIONS: We describe a novel and specific interaction between TAK1 and TBRII which, for the first time, directly links TAK1 to the TGFbeta signalling cascade and potentially explains how TGFbeta signalling in cardiomyocytes mediates a hypertrophic response.
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Authors | Sarah J Watkins, Leon Jonker, Helen M Arthur |
Journal | Cardiovascular research
(Cardiovasc Res)
Vol. 69
Issue 2
Pg. 432-9
(Feb 01 2006)
ISSN: 0008-6363 [Print] England |
PMID | 16360132
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Protein Isoforms
- Receptors, Transforming Growth Factor beta
- Transforming Growth Factor beta
- Protein Serine-Threonine Kinases
- MAP Kinase Kinase Kinases
- MAP kinase kinase kinase 7
- Activin Receptors, Type I
- Receptor, Transforming Growth Factor-beta Type I
- Receptor, Transforming Growth Factor-beta Type II
- TGFBR1 protein, human
- Tgfbr1 protein, mouse
- Tgfbr1 protein, rat
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Topics |
- Activin Receptors, Type I
(metabolism)
- Animals
- Binding, Competitive
- COS Cells
- Cardiomegaly
(metabolism)
- Cell Line
- Chlorocebus aethiops
- Humans
- Immunoprecipitation
- MAP Kinase Kinase Kinases
(metabolism)
- Mice
- Microscopy, Fluorescence
- Myoblasts
(metabolism)
- Myocardium
(metabolism)
- Myocytes, Cardiac
(metabolism)
- Protein Isoforms
(metabolism)
- Protein Serine-Threonine Kinases
- Rats
- Receptor, Transforming Growth Factor-beta Type I
- Receptor, Transforming Growth Factor-beta Type II
- Receptors, Transforming Growth Factor beta
(metabolism)
- Signal Transduction
(physiology)
- Transfection
- Transforming Growth Factor beta
(metabolism)
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