Gene-chip analysis of the effect of tripeptide tyroservatide (YSV) on gene-expression in human hepatocarcinoma BEL-7402 tumors transplanted to nude mice.

Abstract

Tyroservatide (YSV) is a bioactive tripeptide of tyrosyl-seryl-valine. In this study, we studied the effects of YSV on human hepatocarcinoma BEL-7402 tumors transplanted in BALB/c (nu/nu) nude mice, and gene expression in the tumor cells with gene-chip analysis. Results show that YSV significantly inhibits the growth of transplanted human hepatocarcinoma BEL-7402 in nude mice (n = 12) compared with the control group (P < 0.05); with an inhibition rate of 55% at 320 microg/kg/d. Seven hundred eighty-one genes were different between the YSV group and the control group. Fifty-two genes changed in expression level by onefold or more including 37 downregulated genes and 15 upregulated genes. Probably, YSV exhibits a significant antitumor activity by inhibiting the expression of tumor cells histone genes, then damaging tumor cell chromosome and killing tumor cells.

• Authors: Jing Jia, Rong Lu, Chunlei Zhou, Lan Zhao, Zheng Fu, Zhitong Zhu, Li Wang, Minna Zheng, Jing Yang, Yanjun Zhang, Zhi Yao
• Journal: Molecular carcinogenesis (Mol Carcinog) Vol. 45 Issue 3 Pg. 197-203 (Mar 2006) ISSN: 0899-1987 [Print] United States
• PMID: 16353255 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Oligopeptides
• tripeptide tyroservatide

Topics

• Animals
• Carcinoma, Hepatocellular (genetics)
• DNA Primers
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Liver Neoplasms (genetics)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Oligonucleotide Array Sequence Analysis
• Oligopeptides (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Transplantation, Heterologous