Anti-angiogenesis efficacy of the garlic ingredient alliin and antioxidants: role of nitric oxide and p53.

Abstract

Alliin, a compound derived from garlic, demonstrated dose-dependent inhibition of fibroblast growth factor-2 (FGF2)-induced human endothelial cell (EC) tube formation and angiogenesis in the chick chorioallantoic membrane (CAM) model. Additionally, alliin demonstrated potent inhibition of vascular endothelial growth factor (VEGF)-induced angiogenesis in the CAM model. The antioxidant vitamins C and E significantly (P < 0.001) enhanced the inhibitory efficacy of alliin on FGF2-induced EC tube formation and angiogenesis. Alliin significantly increased (P < 0.01) nitric oxide (NO) release into the CAM fluid, which was further enhanced by vitamins C and E. The NO synthesis inhibitor nitro-L-arginine methyl ester (L-NAME) reversed the anti-angiogenesis efficacy of alliin in the CAM model. Vitamins C and E significantly enhanced the anticancer efficacy of alliin in inhibiting colon and fibrosarcoma tumor growth. Alliin significantly inhibited both FGF2 and VEGF secretion from human fibrosarcoma cells in a concentration-dependent manner. Additionally, alliin up-regulated the p53 production in FGF2-stimulated EC. These data indicated a synergistic effect of antioxidants on the anti-angiogenesis and anticancer efficacy of alliin. These data also suggest the implication of cellular NO and p53 as mediators of anti-angiogenesis and anticancer effects of alliin.

• Authors: Ahmed S Mousa, Shaker A Mousa
• Journal: Nutrition and cancer (Nutr Cancer) Vol. 53 Issue 1 Pg. 104-10 ( 2005) ISSN: 0163-5581 [Print] United States
• PMID: 16351512 (Publication Type: Journal Article)

Chemical References

• Angiogenesis Inhibitors
• Antineoplastic Agents
• Antioxidants
• Endothelial Growth Factors
• Tumor Suppressor Protein p53
• Vitamin E
• Nitric Oxide
• alliin
• Cysteine
• Ascorbic Acid

Topics

• Angiogenesis Inhibitors (pharmacology)
• Animals
• Antineoplastic Agents (pharmacology)
• Antioxidants (pharmacology)
• Ascorbic Acid (pharmacology)
• Chick Embryo
• Chorioallantoic Membrane
• Cysteine (analogs & derivatives, pharmacology)
• Dose-Response Relationship, Drug
• Drug Synergism
• Endothelial Growth Factors
• Endothelium, Vascular
• Garlic (chemistry)
• Humans
• Models, Animal
• Nitric Oxide (metabolism, physiology)
• Tumor Cells, Cultured
• Tumor Suppressor Protein p53 (metabolism, physiology)
• Vitamin E (pharmacology)