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Analysis of corrinoids in ovine tissues.

Abstract
Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to estimate the distribution of corrinoids--the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5'-deoxyadenosylcobalamin (ado-cbl), and cobalamin analogues--in these tissues. Samples were taken from either cobalt-deficient or cobalt-replete ewes, and ruminant and pre-ruminant lambs. In liver, ado-cbl predominated, followed by analogues, OH-cbl and me-cbl. Supplementation with either cobalt (ruminant) or vitamin B12 injections (pre-ruminant) increased the amount of ado-cbl and decreased analogues. In blood, OH-cbl predominated, followed by ado-cbl, analogues and me-cbl, respectively. In small intestinal fluid, the distribution from largest to smallest percentage was analogues, ado-cbl, OH-cbl and me-cbl. In faeces, analogues constituted the greatest proportion, followed by OH-cbl, ado-cbl and me-cbl, respectively. Owing to the small sample sizes only cautionary interpretations can be made. In contrast to humans, where me-cbl constitutes the highest proportion of corrinoids in plasma and ado-cbl in the liver, in sheep the amount of ado-cbl was consistently higher than me-cbl in all tissues. This may be due to the higher metabolic need of sheep for ado-cbl due to gluconeogenesis. Analogues and OH-cbl were found in each tissue, contrary to previous postulations. The much higher amount of vitamin B12 in small intestinal fluid compared with faeces indicates that a large proportion of the vitamin is absorbed by the gastro-intestinal tract.
AuthorsR J Kelly, T M Gruner, J M Furlong, A R Sykes
JournalBiomedical chromatography : BMC (Biomed Chromatogr) Vol. 20 Issue 8 Pg. 806-14 (Aug 2006) ISSN: 0269-3879 [Print] England
PMID16345011 (Publication Type: Journal Article)
CopyrightCopyright 2005 John Wiley & Sons, Ltd.
Chemical References
  • Cobamides
  • Corrinoids
  • Cobalt
  • mecobalamin
  • cobamamide
  • Vitamin B 12
  • Hydroxocobalamin
Topics
  • Animals
  • Chromatography, High Pressure Liquid (methods)
  • Cobalt (deficiency, physiology)
  • Cobamides (analysis)
  • Corrinoids (analysis)
  • Feces (chemistry)
  • Female
  • Gastrointestinal Contents (chemistry)
  • Humans
  • Hydroxocobalamin (analysis)
  • Intestine, Small (chemistry)
  • Liver (chemistry)
  • Radioisotope Dilution Technique
  • Sheep
  • Vitamin B 12 (analogs & derivatives, analysis)

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