Cathepsin E is a specific marker of dysplasia in APC mouse intestine.

Abstract

Transcriptional profiling of APC(Min/+) mouse intestinal epithelial tissue has revealed that cathepsin E (catE) manifests high relative expression in adenomas and carcinomas relative to normal epithelium. Real-time RT-PCR data presented previously confirm the presence of catE transcript in APC(Min/+) adenomatous cells compared with samples derived from normal APC(Min/+) and wild-type tissue. At the protein level, strong, highly specific immunohistochemical staining for catE is displayed in dysplastic lesions of APC(Min/+) mice. Using Western immunoblot analyses, it was additionally established that the urine of tumor-bearing mice contains higher levels of the monomeric form of catE than their wild-type counterparts. These results authenticate the relationship between transcript abundance and protein levels in transformed tissue and suggest potential utility for catE as a marker for the inception and progression of intestinal cancers.

• Authors: Lizette Busquets, Hector Guillen, Melanie E DeFord, Mark A Suckow, Rudolph M Navari, Francis J Castellino, Mary Prorok
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 27 Issue 1 Pg. 36-42 ( 2006) ISSN: 1010-4283 [Print] Netherlands
• PMID: 16340248 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright 2006 S. Karger AG, Basel.

Chemical References

• Cathepsin E

Topics

• Adenoma (genetics, pathology)
• Animals
• Blotting, Western
• Carcinoma (genetics, pathology)
• Cathepsin E (analysis, biosynthesis, urine)
• Disease Progression
• Genes, APC
• Immunohistochemistry
• Intestinal Diseases (genetics)
• Intestinal Neoplasms (genetics, pathology)
• Mice
• Reverse Transcriptase Polymerase Chain Reaction