Cloning and characterization of the goadsporin biosynthetic gene cluster from Streptomyces sp. TP-A0584.

Abstract

The biosynthetic gene cluster of goadsporin, a polypeptide antibiotic containing thiazole and oxazole rings, was cloned from Streptomyces sp. TP-A0584. The cluster contains a structural gene, godA, and nine god (goadsporin) genes involved in post-translational modification, immunity and transcriptional regulation. Although the gene organization is similar to typical bacteriocin biosynthetic gene clusters, each goadsporin biosynthetic gene shows low homology to these genes. Goadsporin biosynthesis is initiated by the translation of godA, and the subsequent cyclization, dehydration and acetylation are probably catalysed by godD, godE, godF, godG and godH gene products. godI shows high similarity to the 54 kDa subunit of the signal recognition particle and plays an important role in goadsporin immunity. Furthermore, four goadsporin analogues were produced by site-directed mutagenesis of godA, suggesting that this biosynthesis machinery is used for the heterocyclization of peptides.

• Authors: Hiroyasu Onaka, Mizuho Nakaho, Keiko Hayashi, Yasuhiro Igarashi, Tamotsu Furumai
• Journal: Microbiology (Reading, England) (Microbiology (Reading)) Vol. 151 Issue Pt 12 Pg. 3923-3933 (Dec 2005) ISSN: 1350-0872 [Print] England
• PMID: 16339937 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intercellular Signaling Peptides and Proteins
• Peptides
• Recombinant Proteins
• goadsporin

Topics

• Cloning, Molecular
• Intercellular Signaling Peptides and Proteins
• Molecular Sequence Data
• Multigene Family (genetics)
• Peptides (chemistry, genetics)
• Recombinant Proteins (genetics, metabolism)
• Streptomyces (genetics, metabolism)