Abstract | BACKGROUND: The aim of the present studies was to investigate the changes in concentrations of different forms of thiols in plasma of terminal renal failure patients before and after hemodialysis. METHODS: Total concentrations of thiols, their free forms and the level of their mixed disulfides with proteins were determined with HPLC. RESULTS: In terminal renal failure patients before dialysis, total concentrations of cysteine, homocysteine and cysteinylglycine and their free and protein-bound fractions increased while level of all such forms of glutathione dropped. A single dialysis session caused short-lasting return of concentrations of all forms of thiols to the level equal or close to the control group. The changes observed in non-dialyzed patients were similar to those observed in dialyzed patients before single dialysis procedure. CONCLUSIONS: The obtained results showed severe disturbance of thiol homeostasis in plasma of terminal renal failure patients. The following changes have to be emphasized: (1) high level of free cysteine ( cystine) fraction, (2) strong tendency of homocysteine to form mixed disulfides with proteins, (3) drop of glutathione level. These observations confirm a suggestion that atherogenic action of homocysteine can be a result of S-homocysteinylation and N-homocysteinylation reactions, whereas toxic action of cysteine can result from auto-oxidation reaction.
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Authors | Przemysław J Włodek, Olgierd B Smolenski, Grazyna Chwatko, Małgorzata B Iciek, Andrzej Miłkowski, Edward Bald, Lidia Włodek |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 366
Issue 1-2
Pg. 137-45
(Apr 2006)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 16337615
(Publication Type: Journal Article)
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Chemical References |
- Sulfhydryl Compounds
- Homocysteine
- Glutathione
- Cysteine
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Chromatography, High Pressure Liquid
- Cysteine
(blood)
- Female
- Glutathione
(blood)
- Homeostasis
- Homocysteine
(blood)
- Humans
- Male
- Middle Aged
- Renal Dialysis
- Renal Insufficiency
(blood, therapy)
- Spectrophotometry, Ultraviolet
- Sulfhydryl Compounds
(blood, metabolism)
- Time Factors
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