Identification of class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry.

Abstract

Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.

• Authors: Kenneth L Johnson, Inna G Ovsyannikova, Gregory A Poland, David C Muddiman
• Journal: Journal of proteome research (J Proteome Res) 2005 Nov-Dec Vol. 4 Issue 6 Pg. 2243-9 ISSN: 1535-3893 [Print] United States
• PMID: 16335972 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Cancer Vaccines
• Cations
• HLA Antigens
• HLA-DR Antigens
• HLA-DRB1 Chains
• Hemagglutinins
• Measles Vaccine
• Nucleocapsid Proteins
• Peptides
• Phosphoproteins

Topics

• Amino Acid Sequence
• Cancer Vaccines (chemistry)
• Cations
• Chromatography
• Chromatography, Ion Exchange
• Chromatography, Liquid (methods)
• Databases, Factual
• Databases, Protein
• HLA Antigens (chemistry)
• HLA-DR Antigens (genetics)
• HLA-DRB1 Chains
• Hemagglutinins (chemistry)
• Humans
• Leukocytes, Mononuclear (metabolism)
• Mass Spectrometry (methods)
• Measles Vaccine (chemistry)
• Measles virus (genetics)
• Molecular Sequence Data
• Nucleocapsid Proteins (chemistry)
• Peptides (chemistry, genetics)
• Phosphoproteins (chemistry)
• Proteomics (methods)
• Sequence Homology, Amino Acid
• Time Factors