Abstract |
Poorly soluble photodynamic therapy ( PDT) agent, meso-tetratphenylporphine (TPP), was effectively solubilized using non-targeted and tumor-targeted polymeric micelles prepared of polyethylene glycol/phosphatidyl ethanolamine conjugate ( PEG-PE). Encapsulation of TPP into PEG-PE-based micelles and immunomicelles (bearing an anti- cancer monoclonal 2C5 antibody) resulted in significantly improved anticancer effects of the drug at PDT conditions against murine (LLC, B16) and human (MCF-7, BT20) cancer cells in vitro. For this purpose, the cells were incubated for 6 or 18 h with the TPP or TPP-loaded PEG-PE micelles/immunomicelles and then light-irradiated for 30 min. The phototoxic effect depended on the TPP concentration and specific targeting by immunomicelles. An increased level of apoptosis was shown in the PDT-treated cultures. The attachment of the anti- cancer 2C5 antibodies to TPP-loaded micelles provided the maximum level of cell killing at a given time. The results of this study showed that TPP-containing PEG-PE micelles may represent a useful formulation of the photosensitizer for practical PDT.
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Authors | Aruna Roby, Suna Erdogan, Vladimir P Torchilin |
Journal | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
(Eur J Pharm Biopharm)
Vol. 62
Issue 3
Pg. 235-40
(Apr 2006)
ISSN: 0939-6411 [Print] Netherlands |
PMID | 16326084
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antineoplastic Agents
- Excipients
- Micelles
- Photosensitizing Agents
- Porphyrins
- Polyethylene Glycols
- tetraphenylporphine
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Topics |
- Animals
- Antineoplastic Agents
(administration & dosage, pharmacology)
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Drug Delivery Systems
- Drug Stability
- Excipients
- Humans
- Mice
- Micelles
- Particle Size
- Photochemotherapy
- Photosensitizing Agents
(administration & dosage, chemistry, pharmacology)
- Polyethylene Glycols
- Porphyrins
(administration & dosage, chemistry, pharmacology)
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