Elevation of ST6Gal I activity in malignant and transitional tissue in human colorectal cancer.

Abstract

OBJECTIVES:

The aim of the present study was to investigate the activity of CMP-NeuAc:Galbeta(1,4)GlcNAc sialyltransferase (ST6Gal I) in colorectal cancer (CRC).

METHODS:

ST6Gal I activity was determined in healthy, transitional and tumor tissues from the same patient using asialotransferrin and N-acetyllactosamine as acceptors.

RESULTS:

ST6Gal I activities with asialotransferrin (n = 85) and N-acetyllactosamine (n = 40) as acceptors were statistically significantly enhanced in CRC tissue compared with healthy mucosa from the same patient (p = 0.001). Using transitional tissue (n = 27), enhancement versus healthy tissue was observed (p < 0.05). A positive correlation was found between ST6Gal I activity with N-acetyllactosamine and asialotransferrin in healthy (n = 32), tumorous (n = 32) and transitional tissue (n = 27), supporting the fact that the same enzyme was detected using both acceptors. Furthermore, we studied the relationship between some patients' clinicopathological features and ST6Gal I activity. Although the differences were not statistically significant, the levels of ST6Gal I activity in tumorous and transitional tissues varied with the histological grade of the tumor; however, we failed to find a correlation with the AJCC tumor classification.

CONCLUSIONS:

This work reports enhanced ST6Gal I activity in tumor and transitional tissues from CRC patients. However, our overall results suggest that ST6Gal I activity is not indicative of the patient's outcome.

• Authors: Cristina Vázquez-Martín, Emilio Gil-Martín, Almudena Fernández-Briera
• Journal: Oncology (Oncology) Vol. 69 Issue 5 Pg. 436-44 ( 2005) ISSN: 0030-2414 [Print] Switzerland
• PMID: 16319516 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Sugars
• Asialoglycoproteins
• Biomarkers, Tumor
• Transferrin
• asialotransferrins
• N-acetyllactosamine
• Sialyltransferases
• Neuraminidase
• beta-D-Galactoside alpha 2-6-Sialyltransferase

Topics

• Adenocarcinoma (metabolism)
• Aged
• Aged, 80 and over
• Amino Sugars (chemistry)
• Asialoglycoproteins (metabolism)
• Biomarkers, Tumor
• Chromatography, High Pressure Liquid
• Colorectal Neoplasms (genetics, metabolism)
• Disease Progression
• Female
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• Humans
• Intestinal Mucosa (pathology)
• Kinetics
• Male
• Middle Aged
• Neuraminidase (chemistry)
• Sialyltransferases (biosynthesis, genetics)
• Transferrin (analogs & derivatives, metabolism)
• beta-D-Galactoside alpha 2-6-Sialyltransferase