ApoA-I/phosphatidylcholine discs remodels fast-migrating HDL into slow-migrating HDL as characterized by capillary isotachophoresis.

Abstract

OBJECTIVE:

Capillary isotachophoresis (cITP) is a technique for characterizing plasma lipoprotein subfractions according to their electrophoretic charges. We used this technique to examine the mechanism by which apoA-I/phosphatidylcholine (POPC) discs increase pre-beta HDL.

METHODS AND RESULTS:

The cITP analysis was performed using plasma prestained with a lipophilic dye on a Beckman P/ACE MDQ system. Plasma from a patient with lecithin:cholesterol acyltransferase (LCAT) deficiency who had increased apoE-containing HDL was used to characterize the charge distribution of apoA-I/POPC discs. cITP analysis of apoB- and E-depleted plasma of the patient in the presence of apoA-I/POPC discs indicated two major subfractions of apoA-I/POPC discs with mobilities of triglyceride-rich lipoproteins (fast and slow apoA-I). Incubation of whole plasma from a normolipidemic subject in the presence of apoA-I/POPC discs caused a reduction in cITP fast (f)- and intermediate (i)-migrating HDL, and fast and slow apoA-I, and an increase in slow (s)-migrating HDL. The changes in cITP lipoprotein subfractions were not affected by the inhibition of LCAT activity. ApoA-I/POPC discs increased the fractional esterification rate of cholesterol in apoB-depleted plasma.

CONCLUSION:

ApoA-I/POPC discs remodeled cITP fHDL and iHDL to sHDL independent of LCAT activity.

• Authors: Bo Zhang, Shin-ichiro Miura, Ping Fan, Koichiro Kumagai, Kazuma Takeuchi, Yoshinari Uehara, Monica McMahon, Kerry-Anne Rye, Keijiro Saku
• Journal: Atherosclerosis (Atherosclerosis) Vol. 188 Issue 1 Pg. 95-101 (Sep 2006) ISSN: 0021-9150 [Print] Ireland
• PMID: 16307746 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoprotein A-I
• High-Density Lipoproteins, Pre-beta
• Lipoproteins, HDL
• Phosphatidylcholines
• Phosphatidylcholine-Sterol O-Acyltransferase
• 1-palmitoyl-2-oleoylphosphatidylcholine

Topics

• Apolipoprotein A-I (pharmacology)
• Electrophoresis, Capillary (methods)
• High-Density Lipoproteins, Pre-beta (blood)
• Humans
• Lecithin Cholesterol Acyltransferase Deficiency (drug therapy)
• Lipoproteins, HDL (drug effects)
• Monitoring, Physiologic
• Phosphatidylcholine-Sterol O-Acyltransferase (antagonists & inhibitors)
• Phosphatidylcholines (pharmacology)