Abstract | AIMS: The aim of this study was to isolate and characterize bacteriophages against bovine Staphylococcus aureus associated with mastitis. METHODS AND RESULTS: We describe the isolation of two anti-staphylococcal phages namely DW2 and CS1 from farmyard slurry. Both phages were characterized by electron microscopy and restriction analysis and shown to belong to the Siphoviridae family. CS1 and DW2 were lytic for representatives of all three clonal groups of Irish mastitis-associated staphylococci. These phages were compared with the previously characterized Myoviridae phage K. Infusion of a cocktail of all three phages at 10(8) PFU ml(-1) into live cow teats resulted in no detectable increase in somatic cell counts in milks indicating that the phages did not irritate the animal. CONCLUSION: Two new anti-staphylococcal phages CS1 and DW2 were isolated and characterized and tested for immunogenicity in animal teats. SIGNIFICANCE AND IMPACT OF THE STUDY:
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Authors | S O'Flaherty, R P Ross, J Flynn, W J Meaney, G F Fitzgerald, A Coffey |
Journal | Letters in applied microbiology
(Lett Appl Microbiol)
Vol. 41
Issue 6
Pg. 482-6
( 2005)
ISSN: 0266-8254 [Print] England |
PMID | 16305674
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Cattle
- Lysogeny
- Mastitis, Bovine
(immunology, microbiology)
- Pest Control, Biological
- Staphylococcal Infections
(microbiology, veterinary)
- Staphylococcus Phages
(enzymology, genetics, isolation & purification, physiology)
- Staphylococcus aureus
(genetics, metabolism, virology)
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