Vaxfectin enhances immunogenicity and protective efficacy of P. yoelii circumsporozoite DNA vaccines.

Abstract

We evaluated the capacity of the cationic lipid based formulation, Vaxfectin, to enhance the immunogenicity and protective efficacy of DNA-based vaccine regimens in the Plasmodium yoelii murine malaria model. We immunized Balb/c mice with varying doses (0.4-50 microg) of plasmid DNA (pDNA) encoding the P. yoelii circumsporozoite protein (PyCSP), either in a homologous DNA/DNA regimen (D-D) or a heterologous prime-boost DNA-poxvirus regimen (D-V). At the lowest pDNA doses, Vaxfectin substantially enhanced IFA titers, ELISPOT frequencies, and protective efficacy. Clinical trials of pDNA vaccines have often used low pDNA doses based on a per kilogram weight basis. Formulation of pDNA vaccines in Vaxfectin may improve their potency in human clinical trials.

• Authors: Martha Sedegah, William O Rogers, Arnel Belmonte, Maria Belmonte, Glenna Banania, Noelle Patterson, Marilyn Ferrari, David C Kaslow, Daniel J Carucci, Thomas L Richie, Denise L Doolan
• Journal: Vaccine (Vaccine) Vol. 24 Issue 11 Pg. 1921-7 (Mar 10 2006) ISSN: 0264-410X [Print] Netherlands
• PMID: 16298024 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Protozoan
• Malaria Vaccines
• Phosphatidylethanolamines
• Protozoan Proteins
• Vaccines, DNA
• circumsporozoite protein, Protozoan
• vaxfectin

Topics

• Adjuvants, Immunologic (administration & dosage)
• Animals
• Antibodies, Protozoan (blood)
• Female
• Fluorescent Antibody Technique, Indirect
• Humans
• Immunization, Secondary
• Lymphocytes (immunology)
• Malaria (prevention & control)
• Malaria Vaccines (administration & dosage, genetics, immunology)
• Mice
• Mice, Inbred BALB C
• Phosphatidylethanolamines (administration & dosage, pharmacology)
• Plasmodium yoelii (immunology)
• Protozoan Proteins (genetics, immunology)
• Vaccines, DNA (administration & dosage, genetics, immunology)
• Vaccinia virus (genetics)