Enhancement of ultraviolet-induced apoptosis by NF-kappaB decoy oligonucleotides.

Abstract

BACKGROUND:

A decoy strategy utilizing oligonucleotides (ODN) containing the specific binding sequence of a certain transcription factor has been developed and is considered to be a potential new class of antigene therapy. However, the application of this new therapeutic modality to skin diseases has not been fully documented.

OBJECTIVES:

The aim of this work was to examine the effects of the nuclear factor (NF)-kappaB decoy ODN on UV-elicited skin change.

METHODS:

Mouse keratinocyte Pam 212 cells were transfected with NF-kappaB decoy ODN to examine the effects of the decoy ODN on ultraviolet (UV) B-induced apoptosis. Tape-stripped rat dorsal skin was treated with an ointment containing NF-kappaB decoy ODN for the examination of the in vivo impact of the decoy ODN on sunburned cell (SBC) formation and UVB erythema.

RESULTS:

NF-kappaB decoy ODN specifically induced apoptosis of Pam 212 cells and SBC formation was significantly enhanced by topical NF-kappaB decoy ODN ointment, while UV-induced erythema was not affected.

CONCLUSIONS:

These data suggest that enhancement of UV-induced apoptosis by NF-kappaB decoy ODN may play a cancer-preventive role by further eliminating photodamaged keratinocytes.

• Authors: S Yokoyama, H Nakano, T Yamazaki, K Tamai, K Hanada, G Takahashi
• Journal: The British journal of dermatology (Br J Dermatol) Vol. 153 Suppl 2 Pg. 47-51 (Dec 2005) ISSN: 0007-0963 [Print] England
• PMID: 16280021 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hemoglobins
• Melanins
• NF-kappa B
• Oligonucleotides, Antisense

Topics

• Administration, Topical
• Animals
• Apoptosis
• Cell Line
• DNA Fragmentation
• Electrophoretic Mobility Shift Assay
• Genetic Therapy (methods)
• Hemoglobins (analysis)
• Keratinocytes (pathology, radiation effects)
• Melanins (analysis)
• Mice
• NF-kappa B (genetics, metabolism)
• Oligonucleotides, Antisense (administration & dosage)
• Rats
• Sunburn (metabolism, pathology, prevention & control)
• Transfection (methods)
• Ultraviolet Rays (adverse effects)