Abstract |
2-Methyl-2-(2-methylpropenyl)-2,3-dihydronaphthoquinone[2,3-b] furan-4,9-dione (NFD-37) is a synthetic furonaphthoquinone compound. In this study, we determined that NFD-37 could inhibit the lipopolysaccharide (LPS)-induced production of inflammatory mediators in macrophages RAW 264.7. This compound inhibited LPS-induced nitric oxide (NO) or prostaglandin (PG) E2 production in dose-dependent manners, with IC50 values of 7.2 microM and 5.3 microM, respectively. As the positive controls, pyrrolidine dithiocarbamate (30 microM) exhibited a 57% inhibition of NO production, and NS-398 (1 microM) manifested a 48% inhibition of PGE2 production. The inhibitory effects of NFD-37 on NO and PGE2 production were determined to occur in conjunction with the suppression of inducible NO synthase or cyclooxygenase-2 expression. NFD-37 also inhibited the production of LPS-inducible tumor necrosis factor-alpha, interleukin (IL)-1beta and IL-6, at IC50 values of 4.8-8.9 microM. We also determined the anti-inflammatory efficacy of NFD-37 using carrageenin-induced paw edema in experimental mice.
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Authors | Min-Hee Kim, Hyun-Mo Shin, Yong Rok Lee, Eun Yong Chung, Yoon Sook Chang, Kyung Rak Min, Youngsoo Kim |
Journal | Archives of pharmacal research
(Arch Pharm Res)
Vol. 28
Issue 10
Pg. 1170-6
(Oct 2005)
ISSN: 0253-6269 [Print] Korea (South) |
PMID | 16276975
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 2-methyl-2-(2-methylpropenyl)-2,3-dihydronaphtho(2,3-b)furan-4,9-dione
- Furans
- Inflammation Mediators
- Interleukin-1
- Interleukin-6
- Lipopolysaccharides
- Naphthoquinones
- Tumor Necrosis Factor-alpha
- Nitric Oxide
- Nitric Oxide Synthase Type II
- Cyclooxygenase 2
- Dinoprostone
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Topics |
- Animals
- Blotting, Western
- Cell Line
- Cell Survival
(drug effects)
- Cyclooxygenase 2
(genetics, metabolism)
- Dinoprostone
(metabolism)
- Dose-Response Relationship, Drug
- Furans
(chemistry, pharmacology)
- Inflammation Mediators
(metabolism)
- Interleukin-1
(metabolism)
- Interleukin-6
(metabolism)
- Lipopolysaccharides
(pharmacology)
- Macrophages
(cytology, drug effects, metabolism)
- Molecular Structure
- Naphthoquinones
(chemistry, pharmacology)
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type II
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic
(drug effects)
- Tumor Necrosis Factor-alpha
(metabolism)
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