[Construction, genetic stability and protective efficacy of recombinant fowlpox virus expressing hemagglutinin gene of H5N1 subtype avian influenza virus].

Abstract

Construction, genetic stability and protective efficacy of a recombinant fowlpox virus expressing hemagglutinin (HA) gene (rFPV-HA) of H5 subtype avian influenza virus were conducted. The results indicated that the recombinant fowlpox virus was obtained and purified by blue plaque selection. HA expressed by rFPV-HA was identified by indirect immunofluorescence array. The rFPV-HA could express reporter gene LacZ and HA gene after fifteen successive passages in chicken embryo fibroblast. The rFPV-HA induced completely protection of chickens against lethal challenge with homologous avian influenza virus. The results show that rFPV-HA has good genetic stability and antigenicity and should be a useful tool in HPAI control program by preventing illness and death in chickens when the disease spreads widely.

• Authors: Lijun Jia, Daxin Peng, Yanmei Zhang, Hongqi Liu, Xiufan Liu
• Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (Wei Sheng Wu Xue Bao) Vol. 43 Issue 6 Pg. 722-7 (Dec 2003) ISSN: 0001-6209 [Print] China
• PMID: 16276892 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Hemagglutinin Glycoproteins, Influenza Virus
• Influenza Vaccines
• Recombinant Proteins

Topics

• Animals
• Antibodies, Viral (blood)
• Chick Embryo
• Chickens
• Fowlpox virus (genetics, metabolism)
• Gene Expression
• Genetic Engineering
• Hemagglutinin Glycoproteins, Influenza Virus (genetics, immunology)
• Influenza A Virus, H5N1 Subtype (genetics, immunology)
• Influenza Vaccines (administration & dosage, genetics, immunology)
• Influenza in Birds (immunology, prevention & control, virology)
• Poultry Diseases (immunology, prevention & control, virology)
• Random Allocation
• Recombinant Proteins (genetics, immunology)