Identification of 7 alpha-hydroxy-3-oxo-4-cholestenoic acid in chronic subdural hematoma.

Abstract

We detected a novel kind of bile acid in the content of chronic subdural hematoma. This substance was specifically found in chronic subdural hematoma, and not in subdural hygroma, which is pathologically similar except for the lack of capsular membrane. The compound was identified as 7 alpha-hydroxy-3-oxo-4-cholestenoic acid by high performance liquid chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry. The structure was confirmed by the comparison with the chemically synthesized compound. The average contents in chronic subdural hematoma were 658.09 +/- 137.53 ng/ml, while those in normal human plasma were 126.27 +/- 17.73 ng/ml. It was not detected in normal cerebrospinal fluid. The higher level in chronic subdural hematoma than human plasma strongly suggests the local, extrahepatic production of this type of C27 bile acids.

• Authors: K Nagata, K Takakura, T Asano, Y Seyama, H Hirota, N Shigematsu, I Shima, T Kasama, T Shimizu
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1126 Issue 2 Pg. 229-36 (Jun 22 1992) ISSN: 0006-3002 [Print] Netherlands
• PMID: 1627627 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bile Acids and Salts
• Cholestenones
• 7 alpha-hydroxy-3-oxo-4-cholestenoic acid

Topics

• Bile Acids and Salts (blood, cerebrospinal fluid, chemistry, metabolism)
• Cholestenones (blood, cerebrospinal fluid, chemistry, metabolism)
• Chromatography, High Pressure Liquid
• Chronic Disease
• Gas Chromatography-Mass Spectrometry
• Hematoma, Subdural (etiology, metabolism)
• Humans
• Magnetic Resonance Spectroscopy
• Molecular Structure
• Spectrometry, Mass, Fast Atom Bombardment
• Spectrophotometry, Ultraviolet