Abstract |
The L1 cell adhesion molecule was initially identified and characterized in mouse as a cell-surface glycoprotein that mediates neuron-neuron and neuron-Schwann cell adhesion. We have characterized L1 in humans using cDNA structural and mRNA expression analyses. We present the entire coding sequence for human L1, which predicts a 1253-amino acid protein displaying a signal sequence, transmembrane segment, RGD sequence, and potential glycosylation and phosphorylation sites. Nucleotide and deduced amino acid sequence identities between human and mouse L1 are 85% and 87%, respectively. In contrast, the amino acid identity between human L1 and the L1-related molecule chicken Ng-CAM is only 45%. Using Northern blot analyses, a single L1 transcript of 5.5 kb is detected in human fetal brain and in neuroblastoma (IMR-32) and retinoblastoma (Y-79) cell lines. L1 is also expressed in the rhabdomyosarcoma cell lines RD and A-204, which display several muscle characteristics. Two forms of L1, which differ by the presence or absence of a 12-bp cytoplasmic segment, are expressed in both human and mouse. This segment is encoded by a single exon that can be alternately spliced to give rise to the two forms, which appear to be expressed in tissue-specific patterns.
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Authors | R A Reid, J J Hemperly |
Journal | Journal of molecular neuroscience : MN
(J Mol Neurosci)
Vol. 3
Issue 3
Pg. 127-35
( 1992)
ISSN: 0895-8696 [Print] United States |
PMID | 1627459
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Cell Adhesion Molecules, Neuronal
- Leukocyte L1 Antigen Complex
- RNA, Messenger
- DNA
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Adhesion
- Cell Adhesion Molecules, Neuronal
(biosynthesis, genetics)
- DNA
(genetics)
- Gene Expression
- Genes
- Genetic Variation
- Humans
- Leukocyte L1 Antigen Complex
- Mice
(genetics)
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA Splicing
- RNA, Messenger
(metabolism)
- Sequence Homology, Nucleic Acid
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