[Metallothionein and its isoform genes expression in the human pancreatic cancer cell strains and their function].

Abstract

OBJECTIVE:

To compare the expression of metallothionein (MT) genes and proteins in six human pancreatic cancer cell strains and two human pancreatic cancer drug-resistant cell strains and to explore the relationship between the expression of the MT and pancreatic cancer cell chemo-resistance.

METHODS:

Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to determine the MT isoform-specific mRNA, and cadmium/hemoglobin saturation-electrochemistry to determine MT protein levels.

RESULTS:

MT protein expression in the pancreatic cancer cell strains was encoded by MT-1A, MT-1B, MT-1E, MT-1F, MT-1G, MT-1X, and MT-2A genes. The expression of MT proteins was upregulated and MT-1B, MT-1E, MT-1X, MT-2A genes overexpressed in human pancreatic cancer drug-resistant cell lines (P < 0.05).

CONCLUSION:

Expressions of MT proteins and genes correlate with the proliferation and chemoresistance of human pancreatic cancer cell strains.

• Authors: Yong Xie, Yu-pei Zhao, Ge Chen, Chun-hui Yuan, Li-jun Li
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 27 Issue 5 Pg. 619-23 (Oct 2005) ISSN: 1000-503X [Print] China
• PMID: 16274045 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Metallothionein

Topics

• Cell Division (genetics)
• Drug Resistance, Neoplasm (genetics)
• Humans
• Metallothionein (biosynthesis, genetics)
• Pancreatic Neoplasms (genetics, metabolism, pathology)
• RNA, Messenger (genetics)
• Tumor Cells, Cultured