Abstract | BACKGROUND: STUDY DESIGN AND METHODS: This study investigated the effectiveness of Presbyterian Hb in transfusion therapy with LPS-challenged sepsis mouse model. Septic wild-type mice were transfused with RBCs from Presbyterian Hb-carrying mutant mice and wild-type mice. Their survival rates were assessed, and apoptosis of hepatocytes was evaluated. Survival rates of septic Presbyterian mutant mice and the wild-type littermates were also studied. RESULTS: The Presbyterian mutant RBC-transfused septic group survived longer than the wild-type RBC-transfused group. Apoptosis was reduced in the hepatocytes of the former group. Presbyterian mutant mice themselves, however, did not have stronger resistance to LPS-induced sepsis. CONCLUSION: Transfusion of low-affinity Hb-containing RBCs has beneficial effects in septic mice.
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Authors | Fei Huang, Hidetoshi Nojiri, Takahiko Shimizu, Takuji Shirasawa |
Journal | Transfusion
(Transfusion)
Vol. 45
Issue 11
Pg. 1785-90
(Nov 2005)
ISSN: 0041-1132 [Print] United States |
PMID | 16271104
(Publication Type: Journal Article)
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Chemical References |
- Hemoglobins, Abnormal
- Lipopolysaccharides
- hemoglobin Presbyterian
- Oxygen
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Topics |
- Animals
- Apoptosis
- Bacterial Infections
(physiopathology, therapy)
- Binding, Competitive
- Disease Susceptibility
- Endotoxemia
(physiopathology, therapy)
- Erythrocyte Transfusion
- Erythrocytes
(metabolism)
- Hemoglobins, Abnormal
(genetics)
- Hepatocytes
- Humans
- Lipopolysaccharides
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
(blood, genetics, physiology)
- Oxygen
(blood)
- Survival Analysis
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