Abstract |
Equine herpesvirus-1 (EHV-1) is a ubiquitous pathogen of horses, which continues to cause respiratory and neurological disease and abortion, despite the widespread use of vaccines. Cell mediated immunity (CMI) is thought to play a major role in protection against infection with EHV-1. The aim of this study was to characterise the virus-specific CMI response in ponies vaccinated with vP1014, a vaccinia-based construct (NYVAC) coding for the immediate early gene (gene 64) of EHV-1. This gene product is a CTL target protein for an equine MHC class I allele expressed on the A3 haplotype. EHV-primed yearling ponies expressing this haplotype were vaccinated once (n = 1), three (n = 1), or four times (n = 2), and one pony was kept as an unvaccinated control. Cytotoxic T lymphocyte (CTL) activity and interferon gamma (IFN-gamma) synthesis were measured before and after vaccination and challenge infection with EHV-1. Multiple immunisations with vP1014 resulted in increased CTL activity and IFN-gamma synthesis specific for EHV-1 compared with unvaccinated or singly vaccinated ponies. The phenotype of EHV-1 specific T-cells synthesising IFN-gamma was also modified by immunisation. In the unvaccinated pony, the predominant population synthesising IFN-gamma after EHV-1 stimulation was CD8alpha+. In contrast, multiply vaccinated ponies demonstrated an increased proportion of CD8alpha- T-cells synthesising IFN-gamma. The results demonstrated that vaccination with a NYVAC-based construct coding for gene 64 stimulated CMI. This immune response alone did not protect against challenge infection. However, the study does illustrate that vaccinia-based vaccines can stimulate CMI in the horse and may therefore contribute to protection against disease caused by EHV-1.
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Authors | R Paillot, S A Ellis, J M Daly, J C Audonnet, J M Minke, N Davis-Poynter, D Hannant, J H Kydd |
Journal | Vaccine
(Vaccine)
Vol. 24
Issue 10
Pg. 1490-500
(Mar 06 2006)
ISSN: 0264-410X [Print] Netherlands |
PMID | 16269205
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Herpesvirus Vaccines
- Vaccines, DNA
- Interferon-gamma
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Topics |
- Animals
- Antibodies, Viral
(biosynthesis)
- Female
- Genes, Immediate-Early
- Herpesviridae Infections
(prevention & control, veterinary)
- Herpesvirus 1, Equid
(immunology)
- Herpesvirus Vaccines
(immunology)
- Horse Diseases
(prevention & control)
- Horses
- Immunophenotyping
- Interferon-gamma
(biosynthesis)
- Male
- T-Lymphocytes, Cytotoxic
(immunology)
- Vaccination
- Vaccines, DNA
(immunology)
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