Interleukin-5 modulates eosinophil accumulation in allergic guinea pig lung.

Abstract

Based on its involvement in eosinophil biology, interleukin 5 (IL-5) may play a role in the pulmonary eosinophilia associated with allergic reactions. We have examined that hypothesis using a neutralizing antibody to IL-5 in ovalbumin-sensitized guinea pigs challenged with aerosolized antigen. The extent of eosinophilia has been quantitated in bronchoalveolar lavage (BAL) and by histologic evaluation of lung tissue sections. Acute intraperitoneal administration of a rat IgG, monoclonal antibody to murine IL-5 derived from TRFK-5 cells prevented lung and BAL eosinophilia in a dose-dependent fashion at and above 10 micrograms per guinea pig. Treatment with either an experimentally irrelevant, isotype-matched antibody from GL113 cells or with heat-denatured IL-5 antibody was without effect. These studies demonstrate the importance of IL-5 to pulmonary eosinophilia in challenged, allergic guinea pigs.

• Authors: A R Gulbenkian, R W Egan, X Fernandez, H Jones, W Kreutner, T Kung, F Payvandi, L Sullivan, J A Zurcher, A S Watnick
• Journal: The American review of respiratory disease (Am Rev Respir Dis) Vol. 146 Issue 1 Pg. 263-6 (Jul 1992) ISSN: 0003-0805 [Print] United States
• PMID: 1626810 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Interleukin-5
• Ovalbumin

Topics

• Animals
• Antibodies (administration & dosage)
• Bronchoalveolar Lavage Fluid (pathology)
• Eosinophils (pathology)
• Guinea Pigs
• Immunization
• Interleukin-5 (immunology, pharmacology)
• Lung (pathology)
• Male
• Ovalbumin (immunology)
• Respiratory Hypersensitivity (pathology)