Biological and molecular characterization of a canine hemangiosarcoma-derived cell line.

Abstract

Canine hemangiosarcoma (HSA) is a devastating disease. Investigation of novel therapies has been limited by the limited availability of canine HSA-derived cell lines. We report the development of a canine HSA-derived cell line, DEN-HSA, which recapitulates features of angiogenic endothelium. DEN-HSA cells were derived from a spontaneous HSA arising in the kidney of a dog. DEN-HSA displayed surface molecules distinctive of endothelial histogenesis, including factor VIII-related antigen, ICAM-1 and alpha(v)beta3 integrin. In vitro, DEN-HSA formed microvascular tube-like structures on Matrigel, and proliferated in response to a variety of angiogenic growth factors. The cells expressed mRNA and protein specific for bFGF and its receptors, and VEGF and its receptors, among others. DEN-HSA conditioned medium evoked a marked angiogenic response in a murine corneal pocket assay, indicating potent proangiogenic activity of substances secreted by this cell line. This research confirms the DEN-HSA cell line as endothelial in origin, suggests the presence of angiogenic growth factor autocrine loops, and offers the potential to utilize DEN-HSA cells for the study of novel therapies that modulate endothelial proliferation.

• Authors: Douglas H Thamm, Erin B Dickerson, Nasim Akhtar, Rachel Lewis, Robert Auerbach, Stuart C Helfand, E Gregory MacEwen
• Journal: Research in veterinary science (Res Vet Sci) Vol. 81 Issue 1 Pg. 76-86 (Aug 2006) ISSN: 0034-5288 [Print] England
• PMID: 16256156 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenic Proteins
• RNA, Messenger

Topics

• Angiogenic Proteins (analysis, biosynthesis)
• Animals
• Cell Proliferation
• Dogs
• Flow Cytometry
• Hemangiosarcoma (pathology, veterinary)
• Immunohistochemistry
• Kidney Neoplasms (pathology, veterinary)
• Neovascularization, Pathologic
• RNA, Messenger (biosynthesis)
• Tumor Cells, Cultured (metabolism)