High-performance liquid chromatographic determination of vertilmicin in rat plasma using sensitive fluorometric derivatization.

Abstract

A sensitive and reliable high-performance liquid chromatographic method was developed for the determination of vertilmicin in rat plasma. Derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) followed by C(18) reversed-phase chromatography allowed the fluorimetric detection of vertilmicin. Optimal conditions for the derivatization of vertilmicin are described. The limit of quantification was 0.02 mg/L. The pharmacokinetics of vertilmicin was studied in 24 rats following intramuscular injection (i.m.) of different doses (4, 8, 16, 32 mg/kg of body weight). The pharmacokinetic parameter values were estimated by use of 3P97 program. In this study, we assessed the dose proportionality of vertilmicin after single intramuscular injection doses and obtained new information on the pharmacokinetics of the compound.

• Authors: Zhen Liu, Yunfei Sha, Taomin Huang, Bei Yang, Geng-Li Duan
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 828 Issue 1-2 Pg. 2-8 (Dec 15 2005) ISSN: 1570-0232 [Print] Netherlands
• PMID: 16253575 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aminoglycosides
• Fluorenes
• vertilmicin
• 1-(9-fluorenyl)methyl chloroformate

Topics

• Aminoglycosides (blood, chemistry, pharmacokinetics)
• Animals
• Chromatography, High Pressure Liquid (methods)
• Dose-Response Relationship, Drug
• Fluorenes (chemistry)
• Hydrogen-Ion Concentration
• Rats
• Sensitivity and Specificity
• Spectrometry, Fluorescence (methods)