Abstract | BACKGROUND: METHODS: In this study, we defined the levels of gingipain-specific antibodies in GAgP patient sera and examined the ability of gingipain-specific antibodies to facilitate opsonophagocytosis of P. gingivalis by human polymorphonuclear leukocytes (PMNs) using a fluorescent phagocytosis assay. RESULTS: GAgP patient sera possessed elevated levels of P. gingivalis-, arginine-gingipain (Rgp)A-, RgpB-, and lysine-gingipain (Kgp)-specific IgG (Kgp > RgpA > P. gingivalis > RgpB). Adsorption of GAgP sera with P. gingivalis whole organisms, RgpA, RgpB, and Kgp conjugated to sepharose beads reduced opsonophagocytosis of P. gingivalis by PMNs. CONCLUSIONS: Our studies demonstrate that GAgP patient sera possess elevated levels of P. gingivalis- and gingipain-specific IgG. Furthermore, we show that gingipain antibodies promote uptake of P. gingivalis by PMNs, and our data suggest that gingipain-specific antibodies may be important for the control of P. gingivalis infections.
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Authors | Frank C Gibson 3rd, Juan Savelli, Thomas E Van Dyke, Caroline Attardo Genco |
Journal | Journal of periodontology
(J Periodontol)
Vol. 76
Issue 10
Pg. 1629-36
(Oct 2005)
ISSN: 0022-3492 [Print] United States |
PMID | 16253083
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Adhesins, Bacterial
- Antibodies, Bacterial
- Gingipain Cysteine Endopeptidases
- Immunoglobulin G
- Opsonin Proteins
- Cysteine Endopeptidases
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Topics |
- Adhesins, Bacterial
(immunology)
- Adult
- Antibodies, Bacterial
(blood)
- Case-Control Studies
- Cysteine Endopeptidases
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Female
- Gingipain Cysteine Endopeptidases
- Humans
- Immunoglobulin G
(blood)
- Male
- Neutrophils
(physiology)
- Opsonin Proteins
(blood)
- Periodontitis
(blood, immunology, microbiology)
- Phagocytosis
(physiology)
- Porphyromonas gingivalis
(enzymology, immunology)
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