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Gingipain-specific IgG in the sera of patients with periodontal disease is necessary for opsonophagocytosis of Porphyromonas gingivalis.

AbstractBACKGROUND:
Porphyromonas gingivalis is a primary etiologic agent of generalized aggressive periodontitis (GAgP), and gingipains, a group of cysteine proteinases, are critical virulence factors expressed by this organism. GAgP patients develop specific antibodies to gingipains; however, the function of these antibodies in the clearance of P. gingivalis infection is poorly understood.
METHODS:
In this study, we defined the levels of gingipain-specific antibodies in GAgP patient sera and examined the ability of gingipain-specific antibodies to facilitate opsonophagocytosis of P. gingivalis by human polymorphonuclear leukocytes (PMNs) using a fluorescent phagocytosis assay.
RESULTS:
GAgP patient sera possessed elevated levels of P. gingivalis-, arginine-gingipain (Rgp)A-, RgpB-, and lysine-gingipain (Kgp)-specific IgG (Kgp > RgpA > P. gingivalis > RgpB). Adsorption of GAgP sera with P. gingivalis whole organisms, RgpA, RgpB, and Kgp conjugated to sepharose beads reduced opsonophagocytosis of P. gingivalis by PMNs.
CONCLUSIONS:
Our studies demonstrate that GAgP patient sera possess elevated levels of P. gingivalis- and gingipain-specific IgG. Furthermore, we show that gingipain antibodies promote uptake of P. gingivalis by PMNs, and our data suggest that gingipain-specific antibodies may be important for the control of P. gingivalis infections.
AuthorsFrank C Gibson 3rd, Juan Savelli, Thomas E Van Dyke, Caroline Attardo Genco
JournalJournal of periodontology (J Periodontol) Vol. 76 Issue 10 Pg. 1629-36 (Oct 2005) ISSN: 0022-3492 [Print] United States
PMID16253083 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Adhesins, Bacterial
  • Antibodies, Bacterial
  • Gingipain Cysteine Endopeptidases
  • Immunoglobulin G
  • Opsonin Proteins
  • Cysteine Endopeptidases
Topics
  • Adhesins, Bacterial (immunology)
  • Adult
  • Antibodies, Bacterial (blood)
  • Case-Control Studies
  • Cysteine Endopeptidases (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gingipain Cysteine Endopeptidases
  • Humans
  • Immunoglobulin G (blood)
  • Male
  • Neutrophils (physiology)
  • Opsonin Proteins (blood)
  • Periodontitis (blood, immunology, microbiology)
  • Phagocytosis (physiology)
  • Porphyromonas gingivalis (enzymology, immunology)

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