Antineutrophil cytoplasmic autoantibodies and anti-Saccharomyces cerevisiae antibodies in inflammatory bowel diseases.

Abstract

BACKGROUND/AIMS:

Perinuclear antineutrophil cytoplasmic autoantibody is a marker for ulcerative colitis, and anti-Saccharomyces cerevisiae antibody is known to be associated with Crohn's disease. The purpose of this study was to search the value of detecting perinuclear antineutrophil cytoplasmic autoantibody and anti-Saccharomyces cerevisiae antibody for the diagnosis of Turkish inflammatory bowel disease patients.

METHODS:

Serum samples were obtained from 80 patients with ulcerative colitis, 61 patients with Crohn's disease and 40 healthy controls. Determination of both anti-Saccharomyces cerevisiae antibody and antineutrophil cytoplasmic autoantibody was performed with the standardized enzyme-linked immunosorbent assay.

RESULTS:

In cases with ulcerative colitis, 65% tested seropositive for antineutrophil cytoplasmic autoantibody, whereas the controls showed 2.5% positivity. In cases with Crohn's disease, 63.9% tested seropositive for anti-Saccharomyces cerevisiae antibody, whereas the controls showed 2.5% seropositivity. The combination of a positive anti-Saccharomyces cerevisiae antibody test and a negative antineutrophil cytoplasmic autoantibody yielded a sensitivity and specificity of 32.0% and 97.5%, respectively. The combination of a positive perinuclear antineutrophil cytoplasmic autoantibody and a negative anti-Saccharomyces cerevisiae antibody test yielded a sensitivity and specificity of 44.2% and 97.5%, respectively.

CONCLUSIONS:

Both serologic tests may aid in the differential diagnosis of inflammatory bowel disease.

• Authors: Z Mesut Yalin Kiliç, Bilge Tunç, Selime Ayaz, Levent Filik, Semra Aktaş, Erkan Parlak, Aysel Ulker
• Journal: The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology (Turk J Gastroenterol) Vol. 15 Issue 4 Pg. 238-42 (Dec 2004) ISSN: 1300-4948 [Print] Turkey
• PMID: 16249978 (Publication Type: Journal Article)

Chemical References

• Antibodies, Antineutrophil Cytoplasmic
• Antibodies, Fungal

Topics

• Adolescent
• Adult
• Aged
• Antibodies, Antineutrophil Cytoplasmic (blood)
• Antibodies, Fungal (blood)
• Case-Control Studies
• Colitis, Ulcerative (blood, complications, diagnosis)
• Crohn Disease (blood, complications, diagnosis)
• Female
• Humans
• Male
• Middle Aged
• Predictive Value of Tests
• Saccharomyces cerevisiae (immunology)
• Turkey