Abstract | BACKGROUND: METHODS: Rats were randomly divided into two groups: one had free access to food, the other was fasted for 16 h. The portal vein was cannulated, and the liver was removed and perfused in a closed ex vivo system. Two modes of perfusion were applied in each series of rats, fed and fasting. In the ischemia-reperfusion mode, the experiment consisted of perfusion for 15 min, warm ischemia for 60 min, and reperfusion during 60 min. In the nonischemia mode, perfusion was maintained during the 135-min study period. Five rats were included in each experimental condition, yielding a total of 20 rats. Liver enzymes, potassium, glucose, lactate, free radicals, i.e., dienes and trienes, and cytochrome c were analyzed in perfusate samples. The proportion of glycogen in hepatocytes was determined in tissue biopsies. RESULTS: CONCLUSIONS: In fed rats, liver injury was moderate, whereas hepatocytes integrity was notably impaired both after continuous perfusion and warm ischemia in fasting animals. Reduced glycogen store in hepatocytes may explain reduced tolerance.
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Authors | Michaela Stadler, Vincent Nuyens, Laurence Seidel, Adelin Albert, Jean G Boogaerts |
Journal | Anesthesiology
(Anesthesiology)
Vol. 103
Issue 5
Pg. 978-86
(Nov 2005)
ISSN: 0003-3022 [Print] United States |
PMID | 16249672
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Blood Glucose
- Enzymes
- Liver Glycogen
- Lactic Acid
- Glycogen
- Cytochromes c
- Bilirubin
- Potassium
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Topics |
- Animals
- Bilirubin
(blood)
- Biomarkers
- Blood Glucose
(metabolism)
- Cytochromes c
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Enzymes
(blood)
- Female
- Glycogen
(metabolism)
- Hepatectomy
- In Vitro Techniques
- Lactic Acid
(blood)
- Liver
(pathology, physiology)
- Liver Function Tests
- Liver Glycogen
(metabolism)
- Nutritional Status
- Oxidative Stress
(physiology)
- Perfusion
- Potassium
(blood)
- Rats
- Rats, Wistar
- Reperfusion Injury
(metabolism, pathology, physiopathology)
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