Abstract | BACKGROUND: METHODS: The study compared the abundance levels of PAR1 RNA and protein using real-time reverse-transcriptase polymerase chain reaction and immunoblotting in freshly resected prostate tissues from early localized-stage disease (n=9) to those from patients with advanced metastatic disease (n=7). PAR1 expression and localization was evaluated using immunohistochemical staining of prostate specimens with benign prostatic hyperplasia (n=27), early- (n=32) and advanced-stage (n=22) prostate cancer. Association analyses of PAR1 expression with expression of VEGF-family of growth factors, their receptors, and clinicopathological characteristics of the patients were also performed. RESULTS: PAR1 RNA expression in advanced-stage prostate was 2.39-fold higher (P=0.024) and its protein expression was 2.75-fold higher (P=5.89x10(-5)) when compared with early-stage prostate cancer. PAR1 expression was localized to endothelial cells in vascular network of prostate tumor areas. The expression of PAR1 correlated statistically significantly with advanced disease stage (P=0.0006) and pre-operative PSA levels (P=0.005) in these samples. CONCLUSIONS: These findings demonstrate that PAR1 expression is increased in prostate cancer. Its predominant expression in vascular network suggests that it may play a direct and crucial role in angiogenesis and could be a relevant target for therapeutic interventions to control or to prevent disease progression.
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Authors | Varsha Kaushal, Manish Kohli, Richard A Dennis, Eric R Siegel, Walter W Chiles, Perkins Mukunyadzi |
Journal | The Prostate
(Prostate)
Vol. 66
Issue 3
Pg. 273-82
(Feb 15 2006)
ISSN: 0270-4137 [Print] United States |
PMID | 16245281
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright (c) 2005 Wiley-Liss, Inc. |
Chemical References |
- RNA, Messenger
- Receptor, PAR-1
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Blotting, Western
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Neoplasm Staging
- Neovascularization, Pathologic
(genetics, pathology)
- Prostatic Hyperplasia
(genetics, metabolism, pathology)
- Prostatic Neoplasms
(blood supply, genetics, metabolism, pathology)
- RNA, Messenger
(biosynthesis, genetics)
- Receptor, PAR-1
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Statistics, Nonparametric
- Up-Regulation
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